Team:BIT/project AC

From 2013.igem.org

(Difference between revisions)
 
(13 intermediate revisions not shown)
Line 8: Line 8:
{
{
   width: 1024px;
   width: 1024px;
-
  background-color: transparent;
 
   border-style: none;
   border-style: none;
   margin: 0 auto;
   margin: 0 auto;
   padding: 0;
   padding: 0;
 +
  background-color: #000;
}
}
#globalWrapper /* Global Environment (origin item) */
#globalWrapper /* Global Environment (origin item) */
{
{
-
  background-color: #000;
 
-
  background-attachment: fixed;
 
-
  background-position: 50% 0;
 
-
  background-repeat: no-repeat;
 
-
  background-image: url();
 
   overflow: hidden;
   overflow: hidden;
 +
  background-color: #000;
}
}
#top-section
#top-section
Line 34: Line 30:
   display: none;
   display: none;
}
}
 +
#content
#content
{
{
   width: 1024px;
   width: 1024px;
-
  background-color: transparent;
 
   background-attachment: scroll;
   background-attachment: scroll;
   overflow: hidden;
   overflow: hidden;
   border-style: none;
   border-style: none;
 +
  background-color: #000;
}
}
#footer-box
#footer-box
{
{
-
   background-color: transparent;
+
   background-color: #000;
   background-image: none;
   background-image: none;
   border-style: none;
   border-style: none;
Line 57: Line 54:
}
}
 +
a { color: #999; text-decoration: none; font-family: Verdana;}
-
a { color: #000; text-decoration: none; font-family: Verdana; line-height: 1.5;}
+
#content a { color: #000; text-decoration: none; font-family: Verdana;}
-
a:hover { color: #F00; }
+
#content a:hover { color: #F00; }
#header {
#header {
Line 65: Line 63:
width: 1024px;
width: 1024px;
height: 35px;
height: 35px;
-
        background-color: transparent;
 
}
}
#a1 { display:block; width:100px; height: 30px; background-image:url(https://static.igem.org/mediawiki/2013/b/b2/Bita1.png); background-repeat:no-repeat;}
#a1 { display:block; width:100px; height: 30px; background-image:url(https://static.igem.org/mediawiki/2013/b/b2/Bita1.png); background-repeat:no-repeat;}
Line 77: Line 74:
width: 1024px;
width: 1024px;
height: 150px;
height: 150px;
-
background-image:url(https://static.igem.org/mediawiki/2013/5/55/Bitlogo.png);
+
background-image:url(https://static.igem.org/mediawiki/2013/2/23/Bitlogo1.png);
}
}
#menu {
#menu {
Line 132: Line 129:
height: 500px;
height: 500px;
}
}
-
#footer {
+
#footer1 {
margin: 0px auto;
margin: 0px auto;
width: 1024px;
width: 1024px;
height: 100px;
height: 100px;
-
font-family: Verdana, Geneva, sans-serif;
+
        font-family: Verdana, Geneva, sans-serif;
font-size: small;
font-size: small;
text-align: center;
text-align: center;
}
}
 +
#footer1 a {color:#FFF;}
#left {
#left {
background-image:url(https://static.igem.org/mediawiki/2013/5/52/Bitleft.png);
background-image:url(https://static.igem.org/mediawiki/2013/5/52/Bitleft.png);
Line 161: Line 159:
width: 824px;
width: 824px;
margin-left: 200px;
margin-left: 200px;
-
        overflow-x:hidden;
+
overflow-x:hidden;
overflow-y:auto;
overflow-y:auto;
}
}
-
#b1 {color: #09F;}
+
#right a {color: #09F;}
-
#b1:hover { color: #F00;}
+
 
 +
#b1{color: #09F;}
 +
#b1: hover { color: #F00;}
#d1 {
#d1 {
color: #fff;
color: #fff;
Line 184: Line 184:
font-family: Verdana, Geneva, sans-serif;
font-family: Verdana, Geneva, sans-serif;
font-size: medium;
font-size: medium;
 +
        text-align:justify;
color: #CFF;
color: #CFF;
line-height: 25px;
line-height: 25px;
Line 191: Line 192:
padding-left: 20px;
padding-left: 20px;
}
}
-
.t3{ padding-left: 80px; }
+
.t3{ text-align:center; }
 +
.bg{ background-color:black;}
 +
.bg2 {color: #CCC;background-color:black;}
 +
.bg3 {color: #CCC;background-image:url(https://static.igem.org/mediawiki/2013/3/38/Bitbbb.png);}
 +
 
</style>
</style>
</head>
</head>
-
<body>
 
 +
<body>
<div id="header">
<div id="header">
-
   <table width="1024" border="0">
+
   <table width="1024" border="0" class="bg">
         <tr>
         <tr>
           <td width="713"><div><a id="a1" href="https://2013.igem.org/wiki/index.php?title=Team:BIT/project_AC&action=edit"></a></div></td>
           <td width="713"><div><a id="a1" href="https://2013.igem.org/wiki/index.php?title=Team:BIT/project_AC&action=edit"></a></div></td>
-
           <td width="167"><div><a id="a2" href="https://2013.igem.org/Team:BIT"></a></div></td>
+
           <td width="167"><div><a id="a2" href="http://www.igem.org/Main_Page"></a></div></td>
           <td width="130"><div><a id="a3" href="https://2013.igem.org/wiki/index.php?title=Special:UserLogout&returnto=Main_Page"></a></div></td>
           <td width="130"><div><a id="a3" href="https://2013.igem.org/wiki/index.php?title=Special:UserLogout&returnto=Main_Page"></a></div></td>
         </tr>
         </tr>
Line 220: Line 225:
<div id="class">
<div id="class">
-
   <table width="1024" border="0">
+
   <table width="1024" border="0" class="bg">
         <tr>
         <tr>
-
           <td><div id="c1"><ul><li><a href="https://2013.igem.org/Team:BIT/project_A&C">>>MORE</a></li></ul></div></td>
+
           <td><div id="c1"><ul><li><a href="https://2013.igem.org/Team:BIT/project_AC">>>MORE</a></li></ul></div></td>
           <td><div id="c2"><ul><li><a href="https://2013.igem.org/Team:BIT/project_biosensors">>>MORE</a></li></ul></div></td>
           <td><div id="c2"><ul><li><a href="https://2013.igem.org/Team:BIT/project_biosensors">>>MORE</a></li></ul></div></td>
           <td><div id="c3"><ul><li><a href="https://2013.igem.org/Team:BIT/project_microchip">>>MORE</a></li></ul></div></td>
           <td><div id="c3"><ul><li><a href="https://2013.igem.org/Team:BIT/project_microchip">>>MORE</a></li></ul></div></td>
Line 233: Line 238:
     <ul>
     <ul>
<li><a href="https://2013.igem.org/Team:BIT">HOME</a></li>
<li><a href="https://2013.igem.org/Team:BIT">HOME</a></li>
-
<li><a href="https://2013.igem.org/Team:BIT/Team">TEAM</a></li>
+
<li><a href="https://2013.igem.org/Team:BIT/project_biosensors">BIOSENSORS</a></li>
-
<li><a href="https://2013.igem.org/Team:BIT/Project">PROJECT</a></li>
+
<li><a href="https://2013.igem.org/Team:BIT/project_AC">AMPLIFIER</a></li>
-
<li><a href="https://2013.igem.org/Team:BIT/Parts">PARTS</a></li>
+
<li><a href="https://2013.igem.org/Team:BIT/project_microchip">MICRO CHIP</a></li>
-
<li><a href="https://2013.igem.org/Team:BIT/Modeling">MODELING</a></li>
+
<li><a href="https://2013.igem.org/Team:BIT/project_hardware">HARDWARE</a></li>
-
  <li><a href="https://2013.igem.org/Team:BIT/HP">HUMAN PRACTICE</a></li>
+
-
    <li><a href="https://2013.igem.org/Team:BIT/Safety">SAFETY</a></li>
+
-
    <li><a href="https://2013.igem.org/Team:BIT/Notes">NOTES</a></li>
+
</ul>
</ul>
     </div>
     </div>
<div id="right">
<div id="right">
-
  <table width="824" border="0">
+
  <table width="824" border="0" class="bg3">
    <tr>
    <tr>
-
      <td class="t1">Amplifier and Controller</td>
+
      <td width="608" class="t1">Amplifier and Controller</td>
 +
        </tr>
 +
   
 +
<tr>
 +
      <td class="t2"><strong>Amplifier</strong></td>
 +
        </tr>
 +
 +
        <tr>
 +
      <td class="t2">&nbsp;&nbsp;&nbsp;&nbsp;If we give the biosensor an input signal, we will get an output signal, which, however, may not be strong enough for us to detect. Therefore, we have designed an amplifier, which is based on the high activity of T7 promoter, to increase the intensity of the output signal to a specific magnification. We replaced the sequence of the green fluorescent protein of the sensor with the DNA of T7 RNA polymerase to promote the expression of the downstream DNA. Thus we can get stronger fluorescent intensity as expected. </td>
         </tr>
         </tr>
    <tr>
    <tr>
-
      <td class="t2"></td>
+
      <td class="t3"><img src="https://static.igem.org/mediawiki/2013/c/cd/BITA2.jpg"></td>
         </tr>
         </tr>
 +
<tr>
 +
      <td class="t2">&nbsp;&nbsp;&nbsp;&nbsp;As we all know, if the sample of material which the concentration is high enough to be detected is inserted as the input signal, the sensor will be able to “feel” it and produce an output signal. </td>
 +
        </tr>
 +
    <tr>
 +
      <td class="t3"><img src="https://static.igem.org/mediawiki/2013/5/58/BITA3.jpg"></td>
 +
        </tr>
 +
        <tr>
 +
      <td class="t2">&nbsp;&nbsp;&nbsp;&nbsp;However, sometimes, the output is not strong enough for us to detect. To magnify the output signal, we took advantage of the high activity of the T7 promoter. Because the T7 promoter can only be activated by the T7 RNA polymerase, a gene of T7 RNA polymerase and a T7 promoter were inserted at the downstream site of the sensor to get a stronger output. This part is what we call an “amplifier”. </td>
 +
        </tr>
 +
            <tr>
 +
      <td class="t2">&nbsp;</td>
 +
        </tr>
 +
    <tr>
 +
      <td class="t2"><strong>Controller</strong></td>
 +
        </tr>
 +
            <tr>
 +
      <td class="t2">&nbsp;&nbsp;&nbsp;&nbsp;Sometimes we need to enhance the output signal to different degrees. In other words, we want to control the magnification. A “controller” is designed to solve this problem. We inserted a lacO operator between the DNA of T7 RNA polymerase and green fluorescent protein, and added a <i>lacI</i> biobrick in the system. When there is low concentration of IPTG, the <i>lacI</i> will close the <i>lacO</i> to inhibit the expression of gfp DNA. When we add IPTG to the sample, the <i>lacI</i> will be combined with IPTG, and the inhibition of the expression of the downstream DNA will be inhibited. Thus we can control the magnification by controlling the concentration of IPTG.</td>
 +
        </tr>
 +
            <tr>
 +
      <td class="t2">&nbsp;&nbsp;&nbsp;&nbsp;Here we introduced a new part which contains <i>lacI</i> and <i>lacO</i> in the system. The gene of <i>lacI</i> is always expressing, which inhibits the expression of <i>lacO</i>. In this case, even if there is an input signal, no egfp will be expressed.</td>
 +
        </tr>
 +
    <tr>
 +
      <td class="t3"><img  src="https://static.igem.org/mediawiki/2013/d/d6/BITC3.jpg"></td>
 +
        </tr>
 +
        <tr>
 +
      <td class="t2">&nbsp;&nbsp;&nbsp;&nbsp;If we put IPTG in the environment as an inducer, the <i>lacI</i> protein will combine to the IPTG molecules and thus the inhibition will be ceased. As a result, the <i>lacO</i> will be activated, which will lead to the expression of downstream egfp.</td>
 +
        </tr>
 +
    <tr>
 +
      <td class="t3"><img src="https://static.igem.org/mediawiki/2013/0/01/BITC4.jpg"></td>
 +
        </tr>
 +
     
 +
        <tr>
 +
      <td class="t2">&nbsp;&nbsp;&nbsp;&nbsp;With a stable concentration of IPTG, the system will work as expected. When a weak input is given, a weak green fluorescence will be detected, while if the input gets stronger, the intensity of the green fluorescence will increase simultaneously.</td>
 +
        </tr>
 +
        <tr>
 +
      <td class="t3"><img src="https://static.igem.org/mediawiki/2013/6/6c/BITC5.jpg"></td>
 +
        </tr>
 +
<tr>
 +
      <td class="t3"><img src="https://static.igem.org/mediawiki/2013/7/74/BITC6.jpg"></td>
 +
        </tr>
 +
        <tr>
 +
              <td class="t2">&nbsp;&nbsp;&nbsp;&nbsp;Sometimes we would like to control the magnification. This could be realized by regulating the concentration of IPTG. The higher the concentration of IPTG is, the more <i>lacI</i> will be combined. As a result, the expression of downstream egfp will be enhanced.</td>
 +
        </tr>
 +
        <tr>
 +
      <td class="t3"><img  src="https://static.igem.org/mediawiki/2013/9/95/BITC7.jpg"></td>
 +
        </tr>
 +
        <tr>
 +
              <td class="t2">&nbsp;&nbsp;&nbsp;&nbsp;Similarly, we can decrease the magnification by lowering the concentration of IPTG. <br>
 +
This is the part what we call a “controller”.</td>
 +
        </tr>
 +
<tr>
 +
      <td class="t2"><strong>Result</strong></td>
 +
        </tr>
 +
<tr>
 +
      <td class="t3"><img  src="https://static.igem.org/mediawiki/2013/c/c4/BITA4.jpg"></td>
 +
        </tr>         
 +
<tr>
 +
      <td class="t3"><img  src="https://static.igem.org/mediawiki/2013/f/f4/BITA5.jpg"></td>
 +
        </tr>
 +
       </table>
       </table>
</div>
</div>
</div>
</div>
-
<div id="footer">
+
<div id="footer1">
-
   <table width="1024" border="0">
+
   <table width="1024" border="0" class="bg2">
       <tr>
       <tr>
         <td>&nbsp;</td>
         <td>&nbsp;</td>
Line 262: Line 332:
       <tr>
       <tr>
         <td width="110">&nbsp;</td>
         <td width="110">&nbsp;</td>
-
         <td width="790">Beijing Institute of Technology | 5 South Zhongguancun Street, Haidian District</td>
+
         <td width="790">Beijing Institute of Technology | 5 South Zhongguancun Street, Haidian DistrictBeijing, China 100081 </td>
         <td width="110">&nbsp;</td>
         <td width="110">&nbsp;</td>
       </tr>
       </tr>
       <tr>
       <tr>
         <td>&nbsp;</td>
         <td>&nbsp;</td>
-
         <td>Beijing, China 100081  E-mail: yifei0114@bit.edu.cn</td>
+
         <td> E-mail:<a href="mailto:yifei0114@bit.edu.cn"> yifei0114@bit.edu.cn</a></td>
         <td>&nbsp;</td>
         <td>&nbsp;</td>
       </tr>
       </tr>
Line 277: Line 347:
   </table>
   </table>
</div>
</div>
-
 
</body>
</body>
</html>
</html>

Latest revision as of 15:36, 28 October 2013

iGEM BIT

     
  Beijing Institute of Technology | 5 South Zhongguancun Street, Haidian DistrictBeijing, China 100081  
  E-mail: yifei0114@bit.edu.cn  
  Beijing Institute of Technology © 2013 Privacy Policy