Team:Bonn/Project/

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       <div class="subpage-head-link"><a href="javascript:history.back();">Back</a></br> Category: Project</div>
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      <img src="https://static.igem.org/mediawiki/2013/a/a4/Bonn_sponsors_uniBonn.png" class="bottom-sponsor" height="50px" id="sponsor-unibonn" style="margin-right:10px;">
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= Overview =
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      <div class="subpage-head-link"><a href="javascript:history.back();">Back</a></br> <span id="type">Category: <span id="subpage-type">Project</span></span></div>
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            Making <span style="color:#ffffff;font-weight:bold">versatile control of biological machines easily possible</span> for everybody we engineer a system for
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            <span style="color:#ffffff;font-weight:bold">light dependend control of protein activity</span> per protein degradation.</br>
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            Harnessing the <span style="color:#ffffff;font-weight:bold">ClpXP protease system</span> of prokaryotes enables degradation of a peptid tag (ssrA) fused 
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            protein upon induction with the adaptor protein SspB. Using a Split version of SspB, protein degradation is
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            activated through heterodimerisation of both SspB parts.We utilize light dependent heterodimerisation to eventually obtain light inducible degradation. </br>
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            <span style="color:#ffffff;font-weight:bold">Modelling of the light dependency</span> of protein degradation will even make simple and accurate control of
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            protein activity for any desired level of activity possible.</br>
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            As a proof of principle we investigate degradation of the <span style="color:#ffffff;font-weight:bold">fluorescent reporter protein mCherry.</span></br>
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Latest revision as of 02:17, 5 October 2013

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