Team:DTU-Denmark/pBAD SPL

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(pBAD synthetic promoter library)
(pBAD synthetic promoter library)
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== pBAD synthetic promoter library ==
== pBAD synthetic promoter library ==
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As a tool for expressing lethal proteins in ''E.coli'' we made a synthetic promoter library (SPL, [http://dspace.mit.edu/handle/1721.1/60080 RFC 63]) with the pBAD arabinose inducible promoter. The concept was taken from the DTU iGEM team from 2010 [https://2010.igem.org/Team:DTU-Denmark/SPL link].
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As a tool for expressing lethal proteins in ''E.coli'' we made a synthetic promoter library (SPL, [http://dspace.mit.edu/handle/1721.1/60080 RFC 63]) with the pBAD arabinose inducible promoter. The concept was taken from the DTU iGEM team from 2010 [https://2010.igem.org/Team:DTU-Denmark/SPL (link)].
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== Method ==
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=== USER cloning for SPL ===
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== Results ==
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== Example of use ==
[[File:GFP in perimplasm RFP in cytoplasm.png|thumbnail|upright=4|left|alt=Alt text|Caption text]]
[[File:GFP in perimplasm RFP in cytoplasm.png|thumbnail|upright=4|left|alt=Alt text|Caption text]]
[[File:GFP in perimplasm RFP in cytoplasm close up.png|thumbnail|upright=2|left|alt=Alt text|Caption text]]
[[File:GFP in perimplasm RFP in cytoplasm close up.png|thumbnail|upright=2|left|alt=Alt text|Caption text]]
[[File:Graf.PNG|thumbnail|upright=2|left|alt=Alt text|Caption text]]
[[File:Graf.PNG|thumbnail|upright=2|left|alt=Alt text|Caption text]]
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See also [[HelloWorld]].
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Revision as of 19:00, 28 September 2013

pBAD SPL

Contents

pBAD synthetic promoter library

As a tool for expressing lethal proteins in E.coli we made a synthetic promoter library (SPL, [http://dspace.mit.edu/handle/1721.1/60080 RFC 63]) with the pBAD arabinose inducible promoter. The concept was taken from the DTU iGEM team from 2010 (link).

Method

USER cloning for SPL

Results

Example of use

Alt text
Caption text
Alt text
Caption text
Alt text
Caption text

See also HelloWorld.