Team:Grenoble-EMSE-LSU/Project/Biology

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<h3>Response to a Constant Illumination</h3>
<h3>Response to a Constant Illumination</h3>
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<p>Our first goal was to determine whether or not KR-expressing bacterial cells could be killed under illumination with white light, at constant intensity.<br><br></p>
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                                        <p>Our proof of concept experiment was performed using our experimental protocol. Cells from our ON pre culture were re suspended in two different Erlenmeyer flasks, filled with 25 mL M9 medium, supplemented with 200 µg/µL ampicillin, 50 µg/µL kanamycin and 0.05 mM IPTG. The two cell samples were further incubated at 37°C, 200 rpm, while monitoring OD610 and fluorescence at 610 nm. One cell sample was illuminated at maximal intensity from time point 180 min until the end of the kinetic experiment whereas the second one was kept in the dark. Cells were plated on agar plates at each time point, using serial dilutions. Results of the cell plating are shown in Fig. XXX.<br><br></p>
<h3>Comparison with mCherry: Cellular Death is ROS-mediated</h3>
<h3>Comparison with mCherry: Cellular Death is ROS-mediated</h3>

Revision as of 05:00, 3 October 2013

Grenoble-EMSE-LSU, iGEM


Grenoble-EMSE-LSU, iGEM

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