Team:Edinburgh/Project

From 2013.igem.org

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* '''We generated a [[https://2013.igem.org/Team:Edinburgh/Project/Results/Bioethanol_Results|fusion]] of Pyruvate decarboxylase (Pdc) and Alcohol dehydrogense B (AdhB). We characterised it thoroughly and demonstrated that such co-localisation can increase ethanol production at least twofold.'''  
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* '''We generated a [[https://2013.igem.org/Team:Edinburgh/Project/Results/Bioethanol_Results| fusion]] of Pyruvate decarboxylase (Pdc) and Alcohol dehydrogense B (AdhB). We characterised it thoroughly and demonstrated that such co-localisation can increase ethanol production at least twofold.'''  

Revision as of 23:57, 4 October 2013

Results summary

  • We tested the growth of our experimental strain, Bacillus subtilis 168, under various conditions relevant to the work we are going to perform.


  • We tested the Kanamycin resistance conferred by pTG262 in B.subtilis and E.coli.


  • We created an assembly to demonstrate that Fur box is able to repress gene expression upon exposure to high iron concentrations.



  • We cloned a Neisserial Ferric binding protein A (FbpA) as a BioBrick.


  • We generated a [fusion] of Pyruvate decarboxylase (Pdc) and Alcohol dehydrogense B (AdhB). We characterised it thoroughly and demonstrated that such co-localisation can increase ethanol production at least twofold.


  • We cloned SinR and analysed biofilm formation in WT B.subtilis.


  • We had a great summer, and plan to have fun at the Regional Jamboree.