Team:Hong Kong HKUST/Project

From 2013.igem.org

(Difference between revisions)
Line 195: Line 195:
</div>
</div>
</div>
</div>
-
<br><br><br><div id="slide" class="center"><center><h3>FAT BUSTER</h3><br><p>Artificial Futile Cycle</p><br><br><p>While low-fat diet and regular exercise are popular approaches to fight obesity, one easy alternative is simply to increase energy metabolism. <br><br>
+
<br><br><br><div id="slide" class="center"><center><h3>FAT BUSTER</h3>]<p>Artificial Futile Cycle</p><br><br><p>While low-fat diet and regular exercise are popular approaches to fight obesity, one easy alternative is simply to increase energy metabolism. <br><br>
In terms of fat storage, conversion of carbohydrates or protein into fat uses ten times more calories of energy than simply storing fat in a fat cell. This brought us attention to one method of burning calories - increase energy expenditure by converting fat into glucose. However, mammals cannot convert fatty acids into carbohydrate due to lack of glyoxylate enzymes, while plants and bacteria can. We envision to introduce glyoxylate enzymes into mammalian cells and create an artificial futile cycle.<br><br>
In terms of fat storage, conversion of carbohydrates or protein into fat uses ten times more calories of energy than simply storing fat in a fat cell. This brought us attention to one method of burning calories - increase energy expenditure by converting fat into glucose. However, mammals cannot convert fatty acids into carbohydrate due to lack of glyoxylate enzymes, while plants and bacteria can. We envision to introduce glyoxylate enzymes into mammalian cells and create an artificial futile cycle.<br><br>

Revision as of 19:27, 19 September 2013




FAT BUSTER

]

Artificial Futile Cycle



While low-fat diet and regular exercise are popular approaches to fight obesity, one easy alternative is simply to increase energy metabolism.

In terms of fat storage, conversion of carbohydrates or protein into fat uses ten times more calories of energy than simply storing fat in a fat cell. This brought us attention to one method of burning calories - increase energy expenditure by converting fat into glucose. However, mammals cannot convert fatty acids into carbohydrate due to lack of glyoxylate enzymes, while plants and bacteria can. We envision to introduce glyoxylate enzymes into mammalian cells and create an artificial futile cycle.

While the consequence of introducing nonnative cycle unknown, Dean et al. at UCLA recently has introduced glyoxylate shunt into mammalian liver cell to investigate fatty acid metabolism (2009). They observed that although fatty acids could not be converted into glucose in normal mammalian cells, human hepatocytes expressing the glyoxylate shunt have increased fatty acid oxidation and mice expressing the shunt were resistance to diet-induced obesity.

In addition to introducing a constitutive glyoxylate shunt, our team plans to elaborate the UCLA study by introducing inducible system that allows tunable fatty acid uptake by sensing fatty acid concentrations. This inducible system prevents the risk of fatty acid deficiency, while greater fatty acid uptake at high circulating concentrations can be facilitated. Fatty acids uptake will be quantified to compare the activities in wild type cells and cells expressing constitutive and inducible glyoxylate shunt.

We believe the introduction of an inducible glyoxylate shunt will serve as an artificial futile cycle in human liver cell that increases energy expenditure responding to high circulating fatty acid levels. This will help obesity patients increase expenditure of calories and alleviate health complications, including cardiovascular disease, diabetes, and cancers.

Interview

This will be a random text do not pay attention GOD i am so hungry

Presentation

Presentation? Is you okay is you good cause I want know. Whats up moosh army?

Article

Since our wiki team consist of such diverse people, we say: "Why not make the most of it?" And that is how this article was born. Click "Read More" to know about synthetic biology in Korea, Hong Kong, China and Indonesia