Template:Kyoto/Notebook/Sep 11

From 2013.igem.org

(Difference between revisions)
(→Sep 11)
(→Sep 11)
Line 5: Line 5:
!pKaiBC(200p)||10*buffer KOD plus||dNTPs||MgSO4||primer mix(F&R)||KOD plus||MilliQ||total
!pKaiBC(200p)||10*buffer KOD plus||dNTPs||MgSO4||primer mix(F&R)||KOD plus||MilliQ||total
|-
|-
-
| ||2.5||2.5||1.5||1.5||0.5||16|| ||25
+
|0.5||2.5||2.5||1.5||1.5||0.5||16|| ||25
 +
|}
 +
{| class="wikitable"
 +
!PreDenature||Denature||Annealing||Extension||cycle
 +
|-
 +
|　94°C||　98°C||　60°C||　68°C||30
 +
|-
 +
|2min　||10sec　||30sec　||30sec　||
 +
|}
 +
</div>
 +
<div>
 +
{| class="wikitable"
 +
!pKaiBC(400p)||10*buffer KOD plus||dNTPs||MgSO4||primer mix(F&R)||KOD plus||MilliQ||total
 +
|-
 +
|1||2.5||2.5||1.5||1.5||0.5||15.5|| ||25
 +
|}
 +
{| class="wikitable"
 +
!PreDenature||Denature||Annealing||Extension||cycle
 +
|-
 +
|　94&deg;C||　98&deg;C||　60&deg;C||　68&deg;C||30
 +
|-
 +
|2min　||10sec　||30sec　||30sec　||
 +
|}
 +
</div>
 +
<div>
 +
{| class="wikitable"
 +
!pKaiBC(800p)||10*buffer KOD plus||dNTPs||MgSO4||primer mix(F&R)||KOD plus||MilliQ||total
 +
|-
 +
|2||2.5||2.5||1.5||1.5||0.5||14.5|| ||25
 +
|}
 +
{| class="wikitable"
 +
!PreDenature||Denature||Annealing||Extension||cycle
 +
|-
 +
|　94&deg;C||　98&deg;C||　60&deg;C||　68&deg;C||30
 +
|-
 +
|2min　||10sec　||30sec　||30sec　||
 +
|}
 +
</div>
 +
<div>
 +
{| class="wikitable"
 +
!pKaiBC(1ng)||10*buffer KOD plus||dNTPs||MgSO4||primer mix(F&R)||KOD plus||MilliQ||total
 +
|-
 +
|2.5||2.5||2.5||1.5||1.5||0.5||14|| ||25
|}
|}
{| class="wikitable"
{| class="wikitable"

Revision as of 02:57, 24 September 2013

Sep 11

Tatsui

pKaiBC(200p)10*buffer KOD plusdNTPsMgSO4primer mix(F&R)KOD plusMilliQtotal
0.52.52.51.51.50.516 25
PreDenatureDenatureAnnealingExtensioncycle
　94°C　98°C　60°C　68°C30
2min　10sec　30sec　30sec　
pKaiBC(400p)10*buffer KOD plusdNTPsMgSO4primer mix(F&R)KOD plusMilliQtotal
12.52.51.51.50.515.5 25
PreDenatureDenatureAnnealingExtensioncycle
　94°C　98°C　60°C　68°C30
2min　10sec　30sec　30sec　
pKaiBC(800p)10*buffer KOD plusdNTPsMgSO4primer mix(F&R)KOD plusMilliQtotal
22.52.51.51.50.514.5 25
PreDenatureDenatureAnnealingExtensioncycle
　94°C　98°C　60°C　68°C30
2min　10sec　30sec　30sec　
pKaiBC(1ng)10*buffer KOD plusdNTPsMgSO4primer mix(F&R)KOD plusMilliQtotal
2.52.52.51.51.50.514 25
PreDenatureDenatureAnnealingExtensioncycle
　94°C　98°C　60°C　68°C30
2min　10sec　30sec　30sec　
Retrieved from "http://2013.igem.org/Template:Kyoto/Notebook/Sep_11"