Template:Kyoto/Notebook/Sep 11

From 2013.igem.org

(Difference between revisions)
(→Colony PCR)
(→Colony PCR)
Line 59: Line 59:
</div>
</div>
-
===Colony PCR===
 
<div class="experiment">
<div class="experiment">
-
<span class="author">tatsui</span>
+
<span class="author">No name</span>
{| class="wikitable"
{| class="wikitable"
!Sample||base pair
!Sample||base pair
|-
|-
-
|Pcon-lacZ&#945(p3B4k5)|712bp|
+
|Pcon-lacZ&#945(p3B4k5)||712bp
|}
|}
{| class="wikitable"
{| class="wikitable"
!PreDenature||Denature||Annealing||Extension||cycle
!PreDenature||Denature||Annealing||Extension||cycle
|-
|-
-
|　&deg;C||　&deg;C||　&deg;C||　&deg;C||--
+
|　94&deg;C||　94&deg;C||　55&deg;C||　68&deg;C||--
|-
|-
-
|（◎Д◎）　||　||　||　||
+
|5min||30s　||30s　||42s　||
 +
|}
 +
</div>
 +
<div>
 +
{| class="wikitable"
 +
!Sample||base pair
 +
|-
 +
|Pcon-spinach-DT(p3B4k5)||605bp
 +
|}
 +
{| class="wikitable"
 +
!PreDenature||Denature||Annealing||Extension||cycle
 +
|-
 +
|　94&deg;C||　94&deg;C||　55&deg;C||　68&deg;C||--
 +
|-
 +
|5min||30s　||30s　||42s　||
 +
|}
 +
</div>
 +
<div>
 +
{| class="wikitable"
 +
!Sample||base pair
 +
|-
 +
|Pbad\araC-RBS-luxI-DT||605bp
 +
|}
 +
{| class="wikitable"
 +
!PreDenature||Denature||Annealing||Extension||cycle
 +
|-
 +
|　94&deg;C||　94&deg;C||　55&deg;C||　68&deg;C||--
 +
|-
 +
|5min||30s　||30s　||2min　||
|}
|}
</div>
</div>

Revision as of 03:23, 24 September 2013

Sep 11

PCR

Tatsui

pKaiBC(200p)10*buffer KOD plusdNTPsMgSO4primer mix(F&R)KOD plusMilliQtotal
0.52.52.51.51.50.516 25
PreDenatureDenatureAnnealingExtensioncycle
　94°C　98°C　60°C　68°C30
2min　10sec　30sec　30sec　
pKaiBC(400p)10*buffer KOD plusdNTPsMgSO4primer mix(F&R)KOD plusMilliQtotal
12.52.51.51.50.515.5 25
PreDenatureDenatureAnnealingExtensioncycle
　94°C　98°C　60°C　68°C30
2min　10sec　30sec　30sec　
pKaiBC(800p)10*buffer KOD plusdNTPsMgSO4primer mix(F&R)KOD plusMilliQtotal
22.52.51.51.50.514.5 25
PreDenatureDenatureAnnealingExtensioncycle
　94°C　98°C　60°C　68°C30
2min　10sec　30sec　30sec　
pKaiBC(1ng)10*buffer KOD plusdNTPsMgSO4primer mix(F&R)KOD plusMilliQtotal
2.52.52.51.51.50.514 25
PreDenatureDenatureAnnealingExtensioncycle
　94°C　98°C　60°C　68°C30
2min　10sec　30sec　30sec　

No name

Samplebase pair
Pcon-lacZ&#945(p3B4k5)712bp
PreDenatureDenatureAnnealingExtensioncycle
　94°C　94°C　55°C　68°C--
5min30s　30s　42s　
Samplebase pair
Pcon-spinach-DT(p3B4k5)605bp
PreDenatureDenatureAnnealingExtensioncycle
　94°C　94°C　55°C　68°C--
5min30s　30s　42s　
Samplebase pair
Pbad\araC-RBS-luxI-DT605bp
PreDenatureDenatureAnnealingExtensioncycle
　94°C　94°C　55°C　68°C--
5min30s　30s　2min　
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