From 2013.igem.org
(Difference between revisions)
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| ===colony PCR=== | | ===colony PCR=== |
| <div class="experiment"> | | <div class="experiment"> |
- | <span class="author">No name</span> | + | <span class="author">Tatsui</span> |
| {| class="wikitable" | | {| class="wikitable" |
| !Sample||base pair | | !Sample||base pair |
Revision as of 10:08, 24 September 2013
Sep 11
PCR
Tatsui
pKaiBC(200p) | 10*buffer KOD plus | dNTPs | MgSO4 | primer mix(F&R) | KOD plus | MilliQ | total
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0.5 | 2.5 | 2.5 | 1.5 | 1.5 | 0.5 | 16 | | 25
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PreDenature | Denature | Annealing | Extension | cycle
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94°C | 98°C | 60°C | 68°C | 30
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2min | 10sec | 30sec | 30sec |
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pKaiBC(400p) | 10*buffer KOD plus | dNTPs | MgSO4 | primer mix(F&R) | KOD plus | MilliQ | total
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1 | 2.5 | 2.5 | 1.5 | 1.5 | 0.5 | 15.5 | | 25
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PreDenature | Denature | Annealing | Extension | cycle
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94°C | 98°C | 60°C | 68°C | 30
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2min | 10sec | 30sec | 30sec |
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pKaiBC(800p) | 10*buffer KOD plus | dNTPs | MgSO4 | primer mix(F&R) | KOD plus | MilliQ | total
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2 | 2.5 | 2.5 | 1.5 | 1.5 | 0.5 | 14.5 | | 25
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PreDenature | Denature | Annealing | Extension | cycle
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94°C | 98°C | 60°C | 68°C | 30
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2min | 10sec | 30sec | 30sec |
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pKaiBC(1ng) | 10*buffer KOD plus | dNTPs | MgSO4 | primer mix(F&R) | KOD plus | MilliQ | total
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2.5 | 2.5 | 2.5 | 1.5 | 1.5 | 0.5 | 14 | | 25
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PreDenature | Denature | Annealing | Extension | cycle
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94°C | 98°C | 60°C | 68°C | 30
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2min | 10sec | 30sec | 30sec |
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colony PCR
Tatsui
Sample | base pair
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Pcon-lacZα(p3B4k5) | 712bp
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PreDenature | Denature | Annealing | Extension | cycle
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94°C | 94°C | 55°C | 68°C | --
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5min | 30s | 30s | 42s |
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Sample | base pair
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Pcon-spinach-DT(p3B4k5) | 605bp
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PreDenature | Denature | Annealing | Extension | cycle
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94°C | 94°C | 55°C | 68°C | --
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5min | 30s | 30s | 42s |
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Sample | base pair
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Pbad\araC-RBS-luxI-DT | 605bp
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PreDenature | Denature | Annealing | Extension | cycle
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94°C | 94°C | 55°C | 68°C | --
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5min | 30s | 30s | 2min |
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Sample | base pair
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9/9Pcon+RBS-luxI-DT | 1079bp
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PreDenature | Denature | Annealing | Extension | cycle
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94°C | 94°C | 55°C | 68°C | --
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50sec | 30sec | 30sec | 1min |
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Sample | base pair
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9/10Pcon+sptamer12_1R-DT | 584bp
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PreDenature | Denature | Annealing | Extension | cycle
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94°C | 94°C | 55°C | 68°C | --
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50sec | 30sec | 30sec | 1min |
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Sample | base pair
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9/10DT+Pcon-pT181 attenuater 1 | 781bp
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PreDenature | Denature | Annealing | Extension | cycle
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94°C | 94°C | 55°C | 68°C | --
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50sec | 30sec | 30sec | 1min |
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Sample | base pair
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9/10Fusion1 attenuater(p3B1C3)-1 | 638bp
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PreDenature | Denature | Annealing | Extension | cycle
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94°C | 94°C | 55°C | 68°C | --
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50sec | 30sec | 30sec | 1min |
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