Team:Freiburg/Project/modeling/crRNA/js
From 2013.igem.org
(Difference between revisions)
Line 1: | Line 1: | ||
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<html> | <html> | ||
<head> | <head> | ||
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<script type="text/javascript" src="script.js"></script> | <script type="text/javascript" src="script.js"></script> | ||
<SCRIPT LANGUAGE="JavaScript"> | <SCRIPT LANGUAGE="JavaScript"> | ||
+ | |||
+ | jQuery( document ).ready( function() { | ||
+ | jQuery( ok ).on( 'click', function() { | ||
+ | // Note: you really shouldn't be polluting the global namespace with your functions. | ||
+ | // Use a more object-oriented approach, i.e. create a MyExtension object and then call | ||
+ | // MyExtension.hideTableRows(); here | ||
+ | CrispR(form); | ||
+ | } ); | ||
+ | } ); | ||
+ | |||
+ | |||
function CrispR (form) { | function CrispR (form) { | ||
var TestVar = form.Sequenz.value; | var TestVar = form.Sequenz.value; |
Revision as of 20:23, 26 September 2013
crRNA-Design
This tool helps you to design a crRNA-insert for pX334a. The oligos contain overhangs which fit to the BbsI-overhangs by pX334a.
For repression of gene transcription by targeting the coding sequence
it´s crucial to target the non template (= coding) DNA strand.
Therefore the oligos must be designed as
follows:
- Search at your desired target sequence for a CCN (reverse complement of the PAM sequence) at the coding strand.
- Extract the following (3') 30 nucleotides.
- Extract the reverse complement.
- Add AAAC at the 5' end and GT at the 3' end. This will be your fist oligo.
- Take the sequence from step 2 and add TAAAAC at the 5' end. This will be your second oligo.