Team:Bonn/Project/

From 2013.igem.org

(Difference between revisions)
(Overview)
(Overview)
Line 10: Line 10:
<html>
<html>
         <div class="subpage-text">
         <div class="subpage-text">
-
             Making <span style="color:#ffffff;font-weight:bold">versatile control of biological machines easily possible</span> for everybody we engineer a system for  
+
             Making versatile control of biological machines easily possible for everybody we engineer a system for  
-
             <span style="color:#ffffff;font-weight:bold">light dependend control of protein activity</span> per protein degradation.</br>
+
             light dependend control of protein activity per protein degradation.</br>
-
             Harnessing the <span style="color:#ffffff;font-weight:bold">ClpXP protease system</span> of prokaryotes enables degradation of a peptid tag (ssrA) fused   
+
             Harnessing the ClpXP protease system of prokaryotes enables degradation of a peptid tag (ssrA) fused   
             protein upon induction with the adaptor protein SspB. Using a Split version of SspB, protein degradation is  
             protein upon induction with the adaptor protein SspB. Using a Split version of SspB, protein degradation is  
             activated through heterodimerisation of both SspB parts.We utilize light dependent heterodimerisation to eventually obtain light inducible degradation. </br>
             activated through heterodimerisation of both SspB parts.We utilize light dependent heterodimerisation to eventually obtain light inducible degradation. </br>
-
             <span style="color:#ffffff;font-weight:bold">Modelling of the light dependency</span> of protein degradation will even make simple and accurate control of  
+
             Modelling of the light dependency of protein degradation will even make simple and accurate control of  
             protein activity for any desired level of activity possible.</br>
             protein activity for any desired level of activity possible.</br>
-
             As a proof of principle we investigate degradation of the <span style="color:#ffffff;font-weight:bold">fluorescent reporter protein mCherry.</span></br>
+
             As a proof of principle we investigate degradation of the fluorescent reporter protein mCherry.</br>
         </br>
         </br>
         </div>
         </div>

Revision as of 18:55, 25 June 2013

Overview

Making versatile control of biological machines easily possible for everybody we engineer a system for light dependend control of protein activity per protein degradation.
Harnessing the ClpXP protease system of prokaryotes enables degradation of a peptid tag (ssrA) fused protein upon induction with the adaptor protein SspB. Using a Split version of SspB, protein degradation is activated through heterodimerisation of both SspB parts.We utilize light dependent heterodimerisation to eventually obtain light inducible degradation.
Modelling of the light dependency of protein degradation will even make simple and accurate control of protein activity for any desired level of activity possible.
As a proof of principle we investigate degradation of the fluorescent reporter protein mCherry.

Retrieved from "http://2013.igem.org/Team:Bonn/Project/"