Team:SJTU-BioX-Shanghai
From 2013.igem.org
(Difference between revisions)
Line 1: | Line 1: | ||
- | {{Template:13SJTU_header}} | + | ''''''Bold text''''''{{Template:13SJTU_header}} |
<html> | <html> | ||
<body> | <body> | ||
Line 16: | Line 16: | ||
<li ><a href="/Team:SJTU-BioX-Shanghai/Project">Overview</a> | <li ><a href="/Team:SJTU-BioX-Shanghai/Project">Overview</a> | ||
</li> | </li> | ||
- | <li ><a href="/Team:SJTU-BioX-Shanghai/Project/Luminous_device/Design"> | + | <li ><a href="/Team:SJTU-BioX-Shanghai/Project/Luminous_device/Design">Luminous System</a> |
</li> | </li> | ||
- | <li ><a href="/Team:SJTU-BioX-Shanghai/Project/Light_sensor/Red">Light | + | <li ><a href="/Team:SJTU-BioX-Shanghai/Project/Light_sensor/Red">Light Sensors</a> |
</li> | </li> | ||
- | <li ><a href="/Team:SJTU-BioX-Shanghai/Project/ | + | <li ><a href="/Team:SJTU-BioX-Shanghai/Project/Regulator/Design_criteria">Regulator-CRISPRi</a> |
- | + | ||
- | + | ||
</li> | </li> | ||
+ | |||
</ul> | </ul> | ||
</li> | </li> | ||
- | <li><a href="/Team:SJTU-BioX-Shanghai/Modeling">Modeling</a><li> | + | <li><a href="/Team:SJTU-BioX-Shanghai/Modeling">Modeling</a> |
+ | <ul> | ||
+ | <li><a href="/Team:SJTU-BioX-Shanghai/Modeling/Light_sensor_characteristic">Light sensor characteristic</a></li> | ||
+ | <li><a href="/Team:SJTU-BioX-Shanghai/Modeling/Report_gene_prediction">Report gene prediction</a></li> | ||
+ | <li><a href="/Team:SJTU-BioX-Shanghai/Modeling/Metabolize_optimization">Metabolize optimization</a></li> | ||
+ | </ul> | ||
+ | <li> | ||
<li><a href="/Team:SJTU-BioX-Shanghai/Parts">Parts</a></li> | <li><a href="/Team:SJTU-BioX-Shanghai/Parts">Parts</a></li> | ||
+ | <li><a href="/Team:SJTU-BioX-Shanghai/Prospect/CRISPRi-on">Prospect</a> | ||
+ | <ul> | ||
+ | <li><a href="/Team:SJTU-BioX-Shanghai/Prospect/CRISPRi-on">CRISPRi-on</a></li> | ||
+ | <li><a href="/Team:SJTU-BioX-Shanghai/Prospect/Smaller_light_sensors">Samaller Light Sensor</a></li> | ||
+ | <li><a href="/Team:SJTU-BioX-Shanghai/Prospect/Absolutely_automatic">Absolutely Automatic</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
<li><a href="/Team:SJTU-BioX-Shanghai/Team">Team</a> | <li><a href="/Team:SJTU-BioX-Shanghai/Team">Team</a> | ||
<ul> | <ul> | ||
- | <li><a href="/Team:SJTU-BioX-Shanghai/Team">Members</a></li> | + | <li><a href="/Team:SJTU-BioX-Shanghai/Team/members">Members</a></li> |
<li><a href="/Team:SJTU-BioX-Shanghai/Team">Attributions</a></li> | <li><a href="/Team:SJTU-BioX-Shanghai/Team">Attributions</a></li> | ||
</ul> | </ul> | ||
Line 36: | Line 48: | ||
<li><a href="/Team:SJTU-BioX-Shanghai/Notebook/protocol">Notebook</a> | <li><a href="/Team:SJTU-BioX-Shanghai/Notebook/protocol">Notebook</a> | ||
<ul> | <ul> | ||
- | <li><a href="/Team:SJTU-BioX-Shanghai/protocol">Protocol</a> | + | <li><a href="/Team:SJTU-BioX-Shanghai/Notebook/protocol">Protocol</a> |
</li> | </li> | ||
- | <li><a href="/Team:SJTU-BioX-Shanghai/Notebook/ | + | <li><a href="/Team:SJTU-BioX-Shanghai/Notebook/Lab_log/July">Lablog</a> |
</li> | </li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
- | <li><a href="/Team:SJTU-BioX-Shanghai/Consideration/human">Human Practice</a></li> | + | <li><a href="/Team:SJTU-BioX-Shanghai/Consideration/human">Human Practice</a> |
+ | <ul><li><a href="/Team:SJTU-BioX-Shanghai/Consideration/human">BioCraft</a></li> | ||
+ | <li><a href="/Team:SJTU-BioX-Shanghai/Consideration/human/CC">Communications on Campus</a></li> | ||
+ | <li><a href="/Team:SJTU-BioX-Shanghai/Consideration/human/RSB">Reserch on Synthetic Biology</a></li> | ||
+ | <li><a href="/Team:SJTU-BioX-Shanghai/Consideration/human/Cooperation">Cooperation</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
<li><a href="/Team:SJTU-BioX-Shanghai/Consideration/safety">Safety</a></li> | <li><a href="/Team:SJTU-BioX-Shanghai/Consideration/safety">Safety</a></li> | ||
</ul> | </ul> | ||
Line 70: | Line 88: | ||
<div id="abstract-title"><p><strong style="color:rgb(255,102,102); font-size:60px;">A</strong>bstract</p></div> | <div id="abstract-title"><p><strong style="color:rgb(255,102,102); font-size:60px;">A</strong>bstract</p></div> | ||
<div id="abstract-content"> | <div id="abstract-content"> | ||
- | <p> | + | <p>How to regulate an <strong>entire metabolic pathway</strong> <font style="font-style:italic;">in vivo</font>, delicately, <strong>accurately and conveniently</strong>, simultaneously controlling the expression of <strong>several</strong> genes? And how to <strong>optimize metabolic fluxes so as to maximize desired products</strong>? For decades, these questions remain haunting to “bioengineers”(especially synthetic biologists) who would like to have certain metabolites produced in live cells. The difficulty is again raised up when target genes endogenously reside on the <strong>genome</strong>, or have been implemented into the genome.</p> |
- | <p> | + | <p>So this year, our team, SJTU-BioX-Shanghai, is dedicated to solve the problem. By integrating <strong>CRISPRi</strong>, the newly developed expression interference tool, with three <strong>light sensors</strong> (namely <font color=red>red light sensor</font>, <font color=green>green light sensor</font> and <font color=blue>blue light sensor</font>), we expect to provide a <strong>versatile</strong> platform on which researchers are able to quantitatively adjust the expression of any three enzymes in whatever pathway – to <strong>change the target, simply change the small guide RNA</strong> (sgRNA)!</p> |
- | <p> | + | <p>In addition, with the <strong>luminous device and accompanying software</strong>, one could simply enter metabolomics data (catalytic rates of related enzymes) into our User Interface. The software will automatically conduct <strong>flux balance analysis (FBA)</strong>, giving out suggestions for optimal expression value of different enzymes. Then, the expression value would be converted into <strong>intensity of lights</strong> that are finally to be exerted on cell cultures. It is -- just convenient! :)</p> |
</div> | </div> | ||
</div> | </div> | ||
Line 89: | Line 107: | ||
<td style="border-top-width:20px;" align="center"> | <td style="border-top-width:20px;" align="center"> | ||
<div id="main-entry"> | <div id="main-entry"> | ||
- | <div id="entry-cover" style="background-image:url(/wiki/images/0/ | + | <div id="entry-cover" style="background-image:url(/wiki/images/0/02/Luminous_System.png);"> |
<a href="/Team:SJTU-BioX-Shanghai/Project/Luminous_device/Design"> | <a href="/Team:SJTU-BioX-Shanghai/Project/Luminous_device/Design"> | ||
<div id="entry-text-red"> | <div id="entry-text-red"> | ||
- | <p>Luminous | + | <p>Luminous System provide us a fascinating tool to adjust expression </p> |
- | + | ||
</div> | </div> | ||
</a> | </a> | ||
Line 99: | Line 117: | ||
</div> | </div> | ||
<div id="main-entry"> | <div id="main-entry"> | ||
- | <div id="entry-cover" style="background-image:url(/wiki/images/ | + | <div id="entry-cover" style="background-image:url(/wiki/images/4/41/Light_Sensors.png);"> |
</div> | </div> | ||
</div> | </div> | ||
<div id="main-entry"> | <div id="main-entry"> | ||
- | <div id="entry-cover" style="background-image:url(/wiki/images/ | + | <div id="entry-cover" style="background-image:url(/wiki/images/8/8c/Regulator.png);"> |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
</div> | </div> | ||
</div> | </div> | ||
+ | |||
</td> | </td> | ||
</tr> | </tr> | ||
Line 120: | Line 133: | ||
<tr><td style="border-top-width:40px;"><!-- achievements--> | <tr><td style="border-top-width:40px;"><!-- achievements--> | ||
<div id="achievements"> | <div id="achievements"> | ||
- | <div id="achievements-title"></div> | + | <div id="achievements-title"><p><strong style="color:rgb(102,102,255); font-size:60px;">A</strong>chievements</p></div> |
<div id="achievements-content"> | <div id="achievements-content"> | ||
- | <p><strong><img src="/wiki/images/7/73/12SJTU_r.png"><font face="Cambria, serif " size="4" | + | |
- | + | <p><strong><img src="/wiki/images/7/73/12SJTU_r.png"><font face="Cambria, serif " size="4"><font face="Wingdings " size="4"></font><font face="Cambria, serif " size="4"> The breakthrough we made:</font></strong> Redefinition of scaffold in Synthetic Biology by recruiting <i>E.coli’</i>s inner membrane as a natural two-dimensional scaffold. </p> | |
<p><strong><font face="Wingdings " size="4">ü</font><font face="Cambria, serif " size="4"> The system we built:</font></strong> 6 membrane proteins orderly organized on the inner membrane of <i>E.coli</i>, the efficiency of which has been proved by fluorescence complementation assay and biosynthesis experiment.</p> | <p><strong><font face="Wingdings " size="4">ü</font><font face="Cambria, serif " size="4"> The system we built:</font></strong> 6 membrane proteins orderly organized on the inner membrane of <i>E.coli</i>, the efficiency of which has been proved by fluorescence complementation assay and biosynthesis experiment.</p> | ||
<p><strong><font face="Wingdings " size="4">ü</font><font face="Cambria, serif " size="4"> The device we created – Membrane Accelerator:</font></strong> A universal tool that serves to accelerate biochemical reactions in <i>E.coli</i>; Rate of fatty acids synthesis was increased by 24 fold compared to wild-type <i>E.coli </i>and 9 fold compared to that with overexpressed cytoplasmic enzymes.</p> | <p><strong><font face="Wingdings " size="4">ü</font><font face="Cambria, serif " size="4"> The device we created – Membrane Accelerator:</font></strong> A universal tool that serves to accelerate biochemical reactions in <i>E.coli</i>; Rate of fatty acids synthesis was increased by 24 fold compared to wild-type <i>E.coli </i>and 9 fold compared to that with overexpressed cytoplasmic enzymes.</p> | ||
Line 148: | Line 161: | ||
<tr><td style="border-top-width:40px;"> | <tr><td style="border-top-width:40px;"> | ||
<div id="sponsors"> | <div id="sponsors"> | ||
- | <div id="sponsors-title"><div> | + | <div id="sponsors-title"><p><strong style="color:rgb(102,255,102);font-size:60px; ">S</strong>ponsors</p></div> |
+ | <div id="sponsors-content"> | ||
+ | <table class="sponsorstable" cellspacing="20" ;="" align="center"><tbody> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <a href="http://www.sjtu.edu.cn/"> | ||
+ | <img src="/wiki/images/6/6f/12SJTU_SJTU.jpg" style="width: 140px;"> | ||
+ | </a> | ||
+ | </td> | ||
+ | <td> | ||
+ | <a href="http://www.bio-x.cn"> | ||
+ | <img src="/wiki/images/1/1a/12SJTU_biox.png" style="width: 140px;"> | ||
+ | </a></td> | ||
+ | <td> | ||
+ | <a href="http://life.sjtu.edu.cn/"> | ||
+ | <img src="/wiki/images/5/5c/12SJTU_LS.png" style="width: 140px;"> | ||
+ | </a></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <a href="http://www.merckmillipore.com/millipore"> | ||
+ | <img src="/wiki/images/e/ec/11SJTU_merck.jpg" style="width: 140px;"> | ||
+ | </a></td> | ||
+ | <td> | ||
+ | <a href="http://www.genscript.com/"> | ||
+ | <img src="/wiki/images/5/55/12SJTU_genscript_logo.jpg" style="width: 140px;"> | ||
+ | </a></td> | ||
+ | <td> | ||
+ | <a href="http://www.boyuanbio.com/"> | ||
+ | <img src="/wiki/images/0/08/博苑科技logo.jpg" style="width: 140px;"> | ||
+ | </a></td> | ||
+ | </tr></tbody></table> | ||
+ | |||
</div> | </div> | ||
</td></tr> | </td></tr> | ||
Line 166: | Line 212: | ||
- | <div align="center" id="footer"> (c) <a href="" target="_self"> | + | <div align="center" id="footer"> (c) <a href="" target="_self">SJTU_BioX_iGEM2013</a> Powered by LukeLiu</div> |
</div> | </div> | ||
</body> | </body> | ||
</html> | </html> |
Revision as of 19:17, 27 September 2013
'Bold text'