"
COLONPCR Protocol
From 2013.igem.org
(Difference between revisions)
(Created page with "===COLONY PCR Protocol for PCR machines=== #98ºC for '''2 min''' #98ºC for '''15 sec''' #60ºC for '''15 sec''' #72ºC for '''2 min''' #repeat steps 2-4 '''31 times''' #72ºC f...") |
(→COLONY PCR Protocol for PCR machines) |
||
| Line 1: | Line 1: | ||
| + | {{:Team:GeorgiaTech/template}} | ||
===COLONY PCR Protocol for PCR machines=== | ===COLONY PCR Protocol for PCR machines=== | ||
#98ºC for '''2 min''' | #98ºC for '''2 min''' | ||
Revision as of 01:14, 28 September 2013
Main Page
COLONY PCR Protocol for PCR machines
- 98ºC for 2 min
- 98ºC for 15 sec
- 60ºC for 15 sec
- 72ºC for 2 min
- repeat steps 2-4 31 times
- 72ºC for 4 min
- Hold at 4ºC
