Template:Kyoto/Notebook/Aug 31

From 2013.igem.org

(Difference between revisions)
(Gel Extraction)
(Miniprep)
 
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!DNA||concentration[µg/mL]||260/280||260/230  
!DNA||concentration[µg/mL]||260/280||260/230  
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|8/30 Plux-RBS-GFP-DT-(1)||124 ||1.77 ||1.87
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|8/30 Plux-RBS-GFP-DT-(1)||124||1.77||1.87
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|8/30 Plux-RBS-GFP-DT-(2)||213 ||1.75 ||1.52
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|8/30 Plux-RBS-GFP-DT-(2)||213||1.75||1.52
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|8/30 Pbad/araC-RBS-RFP||427 ||1.70 ||1.56
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|8/30 Pbad/araC-RBS-RFP||427||1.70||1.56
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|8/30 Pbad/araC-RBS-RFP||670||1.30||0.83
|8/30 Pbad/araC-RBS-RFP||670||1.30||0.83

Latest revision as of 03:52, 28 September 2013

Contents

Aug 31

Gel Extraction

No name

LaneDNAEnzyme
1100bp ladder--
2----
3RBS-lysis2-(9)EcoRI & SpeI
4
5----
6pSB1C3(1)EcoRI & SpeI
7
8----
9pSB1C3(1)XbaI & PstI
10

Igku Aug30 Gel Extraction(N1).jpg
Igku Aug30 Gel Extraction(N2-1).jpg

LaneDNAEnzyme
1100bp ladder--
2tRNA-SpinachEcoRI & SpeI
3
4----
5pT181 attenuator-(2)EcoRI & SpeI
6
7pT181 attenuator-(2)XbaI & PstI
8
9----
10pT181 antisense-(2)EcoRI & SpeI
11

Igku Aug31 Gel Extraction(N2-2).jpg
Igku Aug31 Gel Extraction(N2-3).jpg

LaneDNAEnzyme
1100bp ladder--
2pT181 antisenseXbaRI & PstI
3
4----
5tetR aptamer 12_1R-(1)EcoRI & SpeI
6

Igku Aug31 Gel Extraction(N3-1).jpg
Igku Aug31 Gel Extraction(N3-2).jpg

Nameconcentration[µg/mL]260/280260/230
RBS-lysis2(EcoRI&SpeI)4.02.690.24
pSB1C3(EcoRI&SpeI)6.02.480.05
pSB1C3(XbaI&PstI)------
tRNA-Spinach------
pT181 attenuator-(2)(EcoRI&SpeI)------
pT181 anttenuator-(2)(SbaI&PstI)------
pT181 antisense-(2)(EcoRI&SpeI)------
pT181 antisense-(2)(XbaI&PstI)------
tetR aptamer 12_1R(EcoRI&SpeI)------

Ligation

Nakamoto and Hirano

stateVectorInserterLigation High ver.2
experiment8/30 pSB1C3 (EcoRI & SpeI)6.68/30 tRNA-Spinach (EcoRI & SpeI)12.53.5
experiment8/30 pSB1C3 (EcoRI & SpeI)6.68/30 pT181 antisense (EcoRI & SpeI)12.53.5
experiment8/30 pSB1C3 (XbaI & Pst)12.58/30 pT181 attenuator (XbaI & PstI)253.5
experiment8/30 pSB1C3 (XbaI & Pst)12.58/30 pT181 antisense (XbaI & PstI)253.5
experiment8/20 DT (EcoRI & XbaI)6.68/30 pT181 attenuator(2) (EcoRI & SpeI)12.53.5
experiment8/20 DT (EcoRI & XbaI)6.68/30 pT181 antisense (EcoRI & SpeI)12.53.5
experiment8/27 DT (EcoRI & XbaI)3.08/30 tRNA-Spinach (EcoRI & SpeI)12.53.5
experiment8/20 DT (EcoRI & XbaI)6.68/30 RBS-lysis2 (EcoRI & SpeI)12.53.5
experiment8/22 J23100 (SpeI & PstI)--RBS-tetR-DT (XbaI & PstI)--3.5
experiment8/22 Plac (SpeI & PstI)8.08/18 RBS-lacZα-DT (XbaI & PstI)103.5

Samples were evaporeted used evaporator into about 7 µL

Miniprep

Kojima and Nakamoto

DNAconcentration[µg/mL]260/280260/230
8/30 Plux-RBS-GFP-DT-(1)1241.771.87
8/30 Plux-RBS-GFP-DT-(2)2131.751.52
8/30 Pbad/araC-RBS-RFP4271.701.56
8/30 Pbad/araC-RBS-RFP6701.300.83
8/30 Pcon-RBS-GFP-DT-Pcon-RBS-luxR-DT-(1)3321.732.04
8/30 Pcon-RBS-GFP-DT-Pcon-RBS-luxR-DT-(2)4781.742.09
8/30 Pcon-RBS-GFP-DT-Pcon-RBS-luxR-DT-(3)1461.772.11
8/30 Pcon-RBS-GFP-DT-Pcon-RBS-luxR-DT-(4)4541.752.19