Team:UNITN-Trento/Notebook/Labposts/07/05

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(Difference between revisions)

Revision as of 08:21, 3 October 2013

{ "date" : "2013-07-02", "author" : "fabio-viola", "title" : "BACILLUS SUBTILIS COMES BACK TO LIFE!", "content" : " in order to propagate B. subtilis from the pellet that we purchased (strain ind- tyr+ B.subtilis ATCC 23857) , first we needed to obtain the perfect medium for its growth. We prepared Nutrient Broth for liquid cultures and Nutrient Broth + Agar for plates, following ATCC PROTOCOLS. So we decided to have them in both a STARCH-added version and a version without starch.
Nutrient agar (100 ml) : 0,8 g nutrient broth: 1,5g Agar; water to 100 ml.
Nutrient agar + starch (100 ml) : 0,8 g nutrient broth: 1,5g Agar; 2ml starch from potato, water up to 100 ml.
Nutrient broth (250 ml): 2 g nutrient broth; 250 ml water.
Nutrient broth + starch (250 ml): 2 g nutrient broth; 5 ml starch; water up to 250 ml;
To propagate di original bacillus pellet, we resuspended it in 1 ml of nutrient broth+starch and put this milliliter in 5 ml of nutrient broth+starch for a final 6 ml mother liquid culture. Then we made several other liquid cultures with different bacteria concentrations from the Mother (for each concentration we made both starch and non-starch liquid cultures). We put all in a shaker at 26 degrees. We plated also the same concentrations in several plates with both Nutrient agar + starch and Nutrient agar alone, and put them at 26. Just out of curiosity we decided to put some plates and some liquid cultures at 37 degrees.", "tags" : " Bacillus subtilis " }

@@ Line 1: / Line 1: @@
 {
-	"date" : "2013-07-22",
+	"date" : "2013-07-02",
-	"author" : "thomas",
+	"author" : "fabio-viola",
-	"title" : "Inocula of AraC-pBAD + EFE + Venus",
+	"title" : "BACILLUS SUBTILIS COMES BACK TO LIFE!",
-	"content" : "<html>This is the part four of <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-18-thomas-michele\">this experiment</a>. I proceeded preparing 5 inocula from the 1:1 and 1:4 ligation product Plates. The inocula were grown overnight at 37&deg;C in thermoshaker.  </html>",
+	"content" : "<html> in order to propagate B. subtilis from the pellet that we purchased (strain ind- tyr+ B.subtilis ATCC 23857) , first we needed to obtain the perfect medium for its growth. We prepared Nutrient Broth for liquid cultures and Nutrient Broth + Agar for plates, following ATCC PROTOCOLS. So we decided to have them in both a STARCH-added version and a version without starch.<br>Nutrient agar (100 ml) : 0,8 g nutrient broth: 1,5g Agar; water to 100 ml.<br>Nutrient agar + starch (100 ml) : 0,8 g nutrient broth: 1,5g Agar; 2ml starch from potato, water up to 100 ml.<br>Nutrient broth (250 ml): 2 g nutrient broth; 250 ml water.<br>Nutrient broth + starch (250 ml): 2 g nutrient broth; 5 ml starch; water up to 250 ml; <br>To propagate di original bacillus pellet, we resuspended it in 1 ml of nutrient broth+starch and put this milliliter in 5 ml of nutrient broth+starch for a final 6 ml mother liquid culture. Then we made several other liquid cultures with different bacteria concentrations from the Mother (for each concentration we made both starch and non-starch liquid cultures). We put all in a shaker at 26 degrees. We plated also the same concentrations in several plates with both Nutrient agar + starch and Nutrient agar alone, and put them at 26. Just out of curiosity we decided to put some plates and some liquid cultures at 37 degrees.</html>",
-	"tags" : "EFE-Venus"
+	"tags" : " Bacillus subtilis "
 }