Template:Team:SydneyUni Australia/Calendar/Events List

From 2013.igem.org

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start: new Date(2013, 9, 23),
start: new Date(2013, 9, 23),
description: '<b>Members:</b> Rob, Hugh, Andrew <br> <b>What we did: </b> Patching and lysing cells from transformation plates for PCR screen and pH-chloroacetate screens. No efficient cloning for whole pathway, only for individual parts with tetR-inducible promoter system. Set-up chloride assay for degradation of DCA by clones containing tetR-p450 and tetR-aldA-dhlB-dhlA.'
description: '<b>Members:</b> Rob, Hugh, Andrew <br> <b>What we did: </b> Patching and lysing cells from transformation plates for PCR screen and pH-chloroacetate screens. No efficient cloning for whole pathway, only for individual parts with tetR-inducible promoter system. Set-up chloride assay for degradation of DCA by clones containing tetR-p450 and tetR-aldA-dhlB-dhlA.'
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{
 
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title: 'Chloride assay',
 
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start: new Date(2013, 9, 24),
 
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description: '<b>Members:</b> Rob, Andrew <br> <b>What we did: </b> Performed chloride assay. Appears there is reasonable degradation of DCA by p450.
 
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'
 
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},
 
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{
 
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title: 'Acetaldehyde growth inhibition',
 
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start: new Date(2013, 9, 25),
 
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description: '<b>Members:</b> Rob, Andrew <br> <b>What we did: </b> Set-up an experiment to confirm aldA expression through growth inhibition of control E. coli in LB-acetaldehyde.'
 
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},
 
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{
 
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title: 'Characterising new parts',
 
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start: new Date(2013, 9, 26),
 
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description: '<b>Members:</b> Rob, Andrew <br> <b>What we did: </b> Another LB-acetaldehyde growth experiment in triplicate with a promising tetR-aldA clone. Incubated tetR-p450 clones with DCA in triplicate to confirm result from the 24th October, after 6 hours incubation, GC was not promising. Set-up an ethene-epoxide assay to confirm p450 expression. '
 
}
}
]
]

Revision as of 04:57, 28 October 2013