"
Team:DTU-Denmark/Notebook/17 July 2013
From 2013.igem.org
(→Procedure) |
(→Procedure) |
||
| Line 16: | Line 16: | ||
- miniprep of RFP in pZA21 and cycAX in pZA21 | - miniprep of RFP in pZA21 and cycAX in pZA21 | ||
| + | |||
| + | - made gycerol stocks from ON cultures supposedly containing RFP in pZA21 and cycAX in pZA21 | ||
- gel of yesterday's PCR (to get AMO with USER endings) -> got the fragment | - gel of yesterday's PCR (to get AMO with USER endings) -> got the fragment | ||
| Line 23: | Line 25: | ||
- PCR to add His-tags to construct 1 (Sec-GFP-RFP) and construct 2 (TAT-GFP-RFP) | - PCR to add His-tags to construct 1 (Sec-GFP-RFP) and construct 2 (TAT-GFP-RFP) | ||
| - | - gel on today's PCR -> construct 1 | + | - gel on today's PCR -> construct 1 has no product, construct 2 has an unkown product with length 900 bp |
- gel of colony PCR to check the inserts of last USER cloning -> empty, but the colonies that should have RFP are coloured red and one colony with supposed cycAX is coloured orange | - gel of colony PCR to check the inserts of last USER cloning -> empty, but the colonies that should have RFP are coloured red and one colony with supposed cycAX is coloured orange | ||
Revision as of 11:55, 18 July 2013
Contents |
208
Main purpose
- Miniprep
Who was in the lab
Gosia, Henrike
Procedure
- miniprep of RFP in pZA21 and cycAX in pZA21
- made gycerol stocks from ON cultures supposedly containing RFP in pZA21 and cycAX in pZA21
- gel of yesterday's PCR (to get AMO with USER endings) -> got the fragment
- purified AMO for USER from gel
- PCR to add His-tags to construct 1 (Sec-GFP-RFP) and construct 2 (TAT-GFP-RFP)
- gel on today's PCR -> construct 1 has no product, construct 2 has an unkown product with length 900 bp
- gel of colony PCR to check the inserts of last USER cloning -> empty, but the colonies that should have RFP are coloured red and one colony with supposed cycAX is coloured orange
