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Exeter/24 July 2013
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(Created page with " == Minipreps using the Qiagen kit == We miniprepped: * CcaR * Promoter, RBS x 3 replicates * CcaS * B0034 * Magenta * OmpR promoter * OmpR * YF1 * Lambda inverta * F...")
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Revision as of 11:26, 25 July 2013
Minipreps using the Qiagen kit
We miniprepped:
- CcaR
- Promoter, RBS x 3 replicates
- CcaS
- B0034
- Magenta
- OmpR promoter
- OmpR
- YF1
- Lambda inverta
- Fix J
- Yellow
- Fix J promoter
Glycerol stocks
We made glycerol stocks of what is above, then stored them at -80oC
For future reference, to get glycerol stocks back as plates, take a loop and streak on gel. Has to be taken from frozen glycerol stocks, which are immediately returned to the freezer.
We then made a gel for digests using ethidium bromide.
