Template:Kyoto/Notebook/Sep 17

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Sep 17

Tatsui and Hirano

Sample	medium
Spi-DT	LB medium(+CP)
Pcon-Spi-DT,Pcon-pT181-anti-spi-DT	LB medium(+Amp)

Hirano

Pcon-tet ap-DT(VF)	BigDye	5x buffer	primer(VF)	MilliQ	total
1.3	0.5	1.75	0.5	0.45	10.5

PreDenature	Denature	Annealing	Extension	cycle
96°C	96°C	50°C	60°C	--
2m	10s	5s	4m	30cycles

Pcon-tet ap-DT(VR)	BigDye	5x buffer	primer(VR)	MilliQ	total
1.3	0.5	1.75	0.5	0.45	10.5

PreDenature	Denature	Annealing	Extension	cycle
96°C	96°C	50°C	60°C	--
2m	10s	5s	4m	30cycles

No Name

state	Vector		Inserter		Ligation High ver.2
8/28	name	xx µg/mL	name	xx µg/mL	xx µg/mL
NC	name	xx µg/mL	name	xx µg/mL	xx µg/mL

No Name

Lane	DNA	Enzyme
（＞ω・）b

Name	concentration[µg/mL]	260/280	260/230
（´ω｀）
（ΦωΦ^）