Template:Kyoto/Notebook/Sep 11

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Sep 11

Tatsui

pKaiBC(200p)10*buffer KOD plusdNTPsMgSO4primer mix(F&R)KOD plusMilliQtotal
0.52.52.51.51.50.516 25
PreDenatureDenatureAnnealingExtensioncycle
 94°C 98°C 60°C 68°C30
2min 10sec 30sec 30sec 
pKaiBC(400p)10*buffer KOD plusdNTPsMgSO4primer mix(F&R)KOD plusMilliQtotal
12.52.51.51.50.515.5 25
PreDenatureDenatureAnnealingExtensioncycle
 94°C 98°C 60°C 68°C30
2min 10sec 30sec 30sec 
pKaiBC(800p)10*buffer KOD plusdNTPsMgSO4primer mix(F&R)KOD plusMilliQtotal
22.52.51.51.50.514.5 25
PreDenatureDenatureAnnealingExtensioncycle
 94°C 98°C 60°C 68°C30
2min 10sec 30sec 30sec 
pKaiBC(1ng)10*buffer KOD plusdNTPsMgSO4primer mix(F&R)KOD plusMilliQtotal
2.52.52.51.51.50.514 25
PreDenatureDenatureAnnealingExtensioncycle
 94°C 98°C 60°C 68°C30
2min 10sec 30sec 30sec 

Colony PCR