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Contents 1 Team CRISPR 1.1 July 3 2 Team Caffeine 2.1 June 20

Team CRISPR

July 3

List of BioBricks that are/will be on the way:

Cas9:

• Cas9 - Joe and Anna
• pTet -
• const. promoter + Cas9 - Joe
• promoter + Cas9 + Leader + R + spacer + R (on Kan)
• L + R + spacer + R

Target:

• tracerRNA
• repeat + spacer + repeat - Vanins*2 + underlings
• leader - Ray, Fisal, Dan

Decoy:

• PAM + spacer (on Amp)

Team Caffeine

June 20

Joe, Dan, and I made heat-shock competent cells today - they can be found in the top compartment of the -80º Freezer #3 in the room full of freezers (thanks Cam!). We will be ordering primers (through Diane) to modify the TU Munich caffeine biosynthesis BioBricks we got in the Distribution Kit.

We're making a start - what are the other groups up to?

--grace.yi