Team:Heidelberg/Tyrocidine week16 biobrickmod

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Contents

RFC10 Standardization of Tyc Modules

Primer Design

Amplification of Tyc A

TycA&B1 after PCR
TycA µl
Bacillus 1
AT01 2
AT02 2
Q5 2x Master mix 10
ddH20 5
Cycles temperature [°C] Time [min:s]
1 98 2:00
35 98 0:10
59 0:30
72 1:30
1 72 2:00
1 10 inf


Result There was a band at the right length but we were not able to cut.

Amplification of Tyc B1

A

TycA&B1 after PCR
TycB1 µl
Bacillus 1
AT03 2
AT04 2
Q5 2x Master mix 10
ddH20 5
Cycles temperature [°C] Time [min:s]
1 98 2:00
35 98 0:10
56 0:30
72 1:30
1 72 2:00
1 10 inf

Result no band in gel, empty lane; optimization