Team:UCSF/Project/Background2
From 2013.igem.org
Operation CRISPR: Deploying precision guided tools to target unique species in a complex microbiome
- Introduce a targeting system into a defined mixture of bacteria such that you can select and introduce manipulations without negatively affecting other bacteria.
- Creating easy to transfer pathways or circuits that can produce a multitude of outcomes (killing, repressing, upregulating).
1. Introducing CRISPRi to a bacterial community:
WHY USE CRISPRi?
1. CRISPRi utilizes gRNAs which are highly specific and customizable.
2. In principle it could be used to take advantage of unique DNA sequences to target specific bacterial species.