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04/09/13
From 2013.igem.org
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(→Gel Electrophoresis) |
(→Gel Electrophoresis) |
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*This was done in order to confirm that the digest worked and also to purify the pSB1C3 vector. | *This was done in order to confirm that the digest worked and also to purify the pSB1C3 vector. | ||
[[File:igem_psb1c3_doubledigest_040913.jpg]] | [[File:igem_psb1c3_doubledigest_040913.jpg]] | ||
| + | *From left to right | ||
| + | #Sample 1.1 - track 2 | ||
| + | #Sample 1.2 - track 3 | ||
| + | #Sample 2.1 - track 4 | ||
| + | #Sample 2.2 - track 5 | ||
| + | **The 2 Kb bands are the pSB1C3 backbone | ||
| + | **The 1 Kb bands are the RFP biobrick | ||
| + | The two higher bands in tracks 2 and 3 are plasmids that were not digested. | ||
==Gel purification of the pSB1C3 backbone and RFP biobrick== | ==Gel purification of the pSB1C3 backbone and RFP biobrick== | ||
Revision as of 08:47, 5 September 2013
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Contents |
Transformation results
Gel Electrophoresis
- The double digest (pSB1C3) from yesterday was run on a 1% gel.
- This was done in order to confirm that the digest worked and also to purify the pSB1C3 vector.
- From left to right
- Sample 1.1 - track 2
- Sample 1.2 - track 3
- Sample 2.1 - track 4
- Sample 2.2 - track 5
- The 2 Kb bands are the pSB1C3 backbone
- The 1 Kb bands are the RFP biobrick
The two higher bands in tracks 2 and 3 are plasmids that were not digested.
Gel purification of the pSB1C3 backbone and RFP biobrick
- The Zymoclean Gel DNA recovery Kit was used to perform the purification and its protocol was followed.
- However the elution step was changed to 12ul of elution buffer, and this step was repeated.
