06/08/13

From 2013.igem.org

(Difference between revisions)
 
(3 intermediate revisions not shown)
Line 16: Line 16:
==Running of an agarose gel==
==Running of an agarose gel==
*Running the gel for digests of samples 5.1, 10.1, 10.2 and 10.3 from the previous day.
*Running the gel for digests of samples 5.1, 10.1, 10.2 and 10.3 from the previous day.
 +
*expectation-7kb band, confirming the presence of the limonene biobrick (5kb) and chloramphenicol backbone (2kb)
 +
[[File:igem_single_dig_060813.jpg]]
 +
*The gel shows the presence of 7kb bands for both digests of samples 10.1, 10.2 and the 5.1 sample, which was expected.
 +
*No bands appeared for samples 5.1.
 +
*For samples 10.3 there is a 2kb band, indicating that with the ligation, the chloroamphenicol circulized.
==Making chloroamphenicol agar plates==
==Making chloroamphenicol agar plates==
Line 28: Line 33:
**Pour out onto 20 petri dishes
**Pour out onto 20 petri dishes
**Leave to set on bench
**Leave to set on bench
 +
 +
==Plating out samples==
 +
*Plating out samples 10.1, 10.2, 10.3 and 5.1 onto chloroamphenicol plates.
 +
*Grow overnight at 37C

Latest revision as of 14:31, 6 August 2013

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions

Running of an agarose gel

  • Running the gel for digests of samples 5.1, 10.1, 10.2 and 10.3 from the previous day.
  • expectation-7kb band, confirming the presence of the limonene biobrick (5kb) and chloramphenicol backbone (2kb)

Igem single dig 060813.jpg

  • The gel shows the presence of 7kb bands for both digests of samples 10.1, 10.2 and the 5.1 sample, which was expected.
  • No bands appeared for samples 5.1.
  • For samples 10.3 there is a 2kb band, indicating that with the ligation, the chloroamphenicol circulized.

Making chloroamphenicol agar plates

  • Making additional plates
    • 400ml of agar
      • Melt in microwave
      • 3mins on low -> shake
      • Repeat
      • 2mins on low -> shake
      • Repeat
    • Add 800ul of chloroamphenicol at a concentration of 25 ul/ml
    • Pour out onto 20 petri dishes
    • Leave to set on bench

Plating out samples

  • Plating out samples 10.1, 10.2, 10.3 and 5.1 onto chloroamphenicol plates.
  • Grow overnight at 37C