09/08/13

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Isolating plasmid

  • From overnight culture, took 3ml of culture and centrifuged into pellet of samples 5.1, 10.1, 10.2, 10.3
  • Isolated the plasmid using omega bio-tek Plasmid mini kit I
  • Concentrations from nano drop are shown in the table below
SampleVolume(ul)Conc.(ng/ul)260/280260/230
5.19265.81.871.75
10.18836.91.801.77
10.28948.31.791.98
10.39417.41.841.59


Glycerol stocks

  • Took 750ul from overnight stock and centrifuged
  • Supernatant was removed
  • Pellet resuspended in 375ul of HMFM
  • samples were then frozen at -80

Restriction mapping

  • using isolated plasmid samples 5.1, 10.1, 10.2, 10.3