12/08/13

(Difference between revisions)

Latest revision as of 14:18, 13 August 2013

Home	Team	Official Team Profile	Project	Parts Submitted to the Registry	Modeling	Notebook	Safety	Attributions

Pipetting out sonicated samples every 30mins
- Pipette 4ul of DNA into Son 1-6 every 30mins
  - Dilute 1ul of DNA from Son 1-6 in 9ul of 1xTE buffer to tubes 10xdil 1-6
  - Vortex each dilution thoroughly and centrifuge briefly.
Keep tubes on ice

To tubes 1-6, add 14 ul of sterile water and 5 ul of orange G dye and 1ul of DNA from Dil x10 1-6 appropriately. Centrifuge briefly. Keep on ice until loading.
Use 5 ul of 1kb marker on both ends
Add buffer so that wells in the tray and the tray itself are covered
Run at 80 V for an hour and a half (gel is 1.2%)

@@ Line 1: / Line 1: @@
+<div style="font-family:arial;padding:5px;border-radius:5px;border:5px solid #FF2800;">
+{| style="color:#87EA00;background-color:#FFFFFF;" cellpadding="2" cellspacing="2" border="0" bordercolor="#000000" width="100%" align="center"
+!align="center"|[[Team:Leicester|Home]]
+!align="center"|[[Team:Leicester/Team|Team]]
+!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Leicester Official Team Profile]
+!align="center"|[[Team:Leicester/Project|Project]]
+!align="center"|[[Team:Leicester/Parts|Parts Submitted to the Registry]]
+!align="center"|[[Team:Leicester/Modeling|Modeling]]
+!align="center"|[[Team:Leicester/Notebook|Notebook]]
+!align="center"|[[Team:Leicester/Safety|Safety]]
+!align="center"|[[Team:Leicester/Attributions|Attributions]]
+|}
+</div>
 ==Sonicating herring sperm DNA==
 *Pipetting out sonicated samples every 30mins