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16/08/13
From 2013.igem.org
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*Each was diluted 100 fold to get a concentration in the range of 40-50ng/ul | *Each was diluted 100 fold to get a concentration in the range of 40-50ng/ul | ||
*5ul of DNA was taken from the dilution, to which 10ul of sterile water and 5ul of orange G was added | *5ul of DNA was taken from the dilution, to which 10ul of sterile water and 5ul of orange G was added | ||
| - | *Samples were run against a 1kb ladder and control herring sperm DNA, which was not sonicated | + | *Samples were run against a 1kb ladder and control herring sperm DNA, which was not sonicated. |
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Revision as of 13:40, 16 August 2013
Running the gel from sonication series
- Samples were taken from sonicated tube every 30min intervals, a total of 6 samples.
- Each was diluted 100 fold to get a concentration in the range of 40-50ng/ul
- 5ul of DNA was taken from the dilution, to which 10ul of sterile water and 5ul of orange G was added
- Samples were run against a 1kb ladder and control herring sperm DNA, which was not sonicated.
