16/09/13

From 2013.igem.org

(Difference between revisions)
(Created page with "==Digesting pSB1C3 backbone== *Digesting for ligation with the already digested Tod operon PCR products *Following the iGEM official protocol *Using EcoRI-HF and PstI-HF *Exludin...")
(Digesting pSB1C3 backbone)
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*Using EcoRI-HF and PstI-HF
*Using EcoRI-HF and PstI-HF
*Exluding the DpnI from the master mix and adjusting the volume of water accordingly
*Exluding the DpnI from the master mix and adjusting the volume of water accordingly
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*Protocol found at: [http://parts.igem.org/Help:Protocols/Linearized_Plasmid_Backbones]
+
*Protocol can be found at: [http://parts.igem.org/Help:Protocols/Linearized_Plasmid_Backbones]
==Running the backbone and RFP on gel==
==Running the backbone and RFP on gel==
*on 1% gel to check if DNA was extracted on Friday
*on 1% gel to check if DNA was extracted on Friday

Revision as of 11:21, 16 September 2013

Digesting pSB1C3 backbone

  • Digesting for ligation with the already digested Tod operon PCR products
  • Following the iGEM official protocol
  • Using EcoRI-HF and PstI-HF
  • Exluding the DpnI from the master mix and adjusting the volume of water accordingly
  • Protocol can be found at: [http://parts.igem.org/Help:Protocols/Linearized_Plasmid_Backbones]

Running the backbone and RFP on gel

  • on 1% gel to check if DNA was extracted on Friday