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16/09/13
From 2013.igem.org
(Difference between revisions)
(Created page with "==Digesting pSB1C3 backbone== *Digesting for ligation with the already digested Tod operon PCR products *Following the iGEM official protocol *Using EcoRI-HF and PstI-HF *Exludin...") |
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*Using EcoRI-HF and PstI-HF | *Using EcoRI-HF and PstI-HF | ||
*Exluding the DpnI from the master mix and adjusting the volume of water accordingly | *Exluding the DpnI from the master mix and adjusting the volume of water accordingly | ||
| - | *Protocol found at: [http://parts.igem.org/Help:Protocols/Linearized_Plasmid_Backbones] | + | *Protocol can be found at: [http://parts.igem.org/Help:Protocols/Linearized_Plasmid_Backbones] |
==Running the backbone and RFP on gel== | ==Running the backbone and RFP on gel== | ||
*on 1% gel to check if DNA was extracted on Friday | *on 1% gel to check if DNA was extracted on Friday | ||
Revision as of 11:21, 16 September 2013
Digesting pSB1C3 backbone
- Digesting for ligation with the already digested Tod operon PCR products
- Following the iGEM official protocol
- Using EcoRI-HF and PstI-HF
- Exluding the DpnI from the master mix and adjusting the volume of water accordingly
- Protocol can be found at: [1]
Running the backbone and RFP on gel
- on 1% gel to check if DNA was extracted on Friday
