16/09/13

From 2013.igem.org

Digesting pSB1C3 backbone

• Digesting for ligation with the already digested Tod operon PCR products
• Following the iGEM official protocol
• Using EcoRI-HF and PstI-HF
• Exluding the DpnI from the master mix and adjusting the volume of water accordingly
• Protocol can be found at: [1]

Running the backbone and RFP on gel

• on 1% gel to check if DNA was extracted on Friday