16 July 2013

From 2013.igem.org

1. No colonies grew on ampicillin plates because PCR products were not of appropriate form, and wrong protocol was used for TOPO TA cloning.
2. New LB agar/Ampicillin plates were prepared.
3. It was decided to repeat TOPO TA cloning, but this time with new correct PCR products and with the correct protocol.