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17 July 2013
From 2013.igem.org
Today we decided to amplify DNA from SELEX 11 with PCR. In addition, this will be DNAs will contain A-overhangs in order to be suitable for TOPO TA cloning.
- PCR for making A-overhangs (total 50ul):
- DNA template 10 - 100 ng
- 10xPCR buffer 5ul
- 50mM dNTPs 1ul each
- Water add to final volume of 49ul
- Taq Polymerase 1ul
Include a 7-30 min extension at 72degC after the last cycle
