18/07/13

From 2013.igem.org

Single digest

  • Digesting RFP plasmid again using a single restriction enzyme- XbaI
  • Digesting DNA samples 1-6
  • 200ng of DNA is run
  • Volumes for digest:
Sample nr123456
DNA0.70.70.81.31.22.6
Buffer222222
Water16.316.316.215.715.814.4
XbaI111111
  • Buffer used was cutsmart
  • Incubate samples at 37C for 1h
  • 2ul of orange G dye was added to each samples
  • Samples were then frozen overnight to run on gel on Monday