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19/08/13
From 2013.igem.org
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Denaturing sonicated DNA
- Denaturing DNA by heat shock for 10mins in a 95C heat block
- Adding 5ul of OG dye
Running both denatured and sonicated samples on gel
- For just the sonication samples 1-6, 5ul of OG and 10ul of sterile water was added to 5ul of DNA.
- 5ul of 1kb ladder was used for 3 wells each.
- The last 6 wells after ladder are the denatured DNA samples.
- We were expecting to see fragmentation for the denatured samples.
- There is no significant difference in between the sonicated samples and the sonicated and denatured ones.
- There is a slight decrease in bands depending on the sonication time interval.
- Suggestions are to use ultra-sonification to view the effects focused just on the sample.
