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Exeter/12 September 2013
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Revision as of 11:27, 12 September 2013 by FelicityHeath (Talk | contribs)
Optical Absorption of liquid cultures
Using the liquid cultures that were made yesterday afternoon, today we used a plate reader to measure the absorbance of the cyan and magenta cultures. Cyan was measured every 4 hours as the colour appears to be developing much slower than the others, but the magenta was measured every two hours.
| Liquid culture | Absorbance at 10:00am | Absorbance at 12:00pm | Absorbance at 14:00pm | Absorbance at 16:00pm |
|---|---|---|---|---|
| Cyan no.2 | 0.602 | - | ? | - |
| Cyan no.3 | 0.817 | - | ? | - |
| Cyan V.2 | 0.894 | - | ? | - |
| Cyan V.4 | 0.740 | - | ? | - |
| G block 11 | 0.542 | 0.519 | ? | ? |
| G block 7 | 1.023 | 1.120 | ? | ? |
Transformations
In the afternoon we transformed the ligations from earlier in the week. This was also done on Tuesday, however, we now have fresh competent cells that we used instead.
