Exeter/12 September 2013

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Optical Absorption of liquid cultures

Using the liquid cultures that were made yesterday afternoon, today we used a plate reader to measure the absorbance of the cyan and magenta cultures. Cyan was measured every 4 hours as the colour appears to be developing much slower than the others, but the magenta was measured every two hours.


Liquid culture Absorbance at 10:00am Absorbance at 12:00pm Absorbance at 14:00pm Absorbance at 16:00pm
Cyan no.2 0.602 -  ? -
Cyan no.3 0.817 -  ? -
Cyan V.2 0.894 -  ? -
Cyan V.4 0.740 -  ? -
G block 11 0.542  ?  ?  ?
G block 7 1.023  ?  ?  ?


Transformations

In the afternoon we transformed the ligations from earlier in the week. This was also done on Tuesday, however, we now have fresh competent cells that we used instead.