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Exeter/16 August 2013
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We have three plates of our gBlocks coding for the green light module, Two show good growth, the third appears to have no growth whatsoever. Liquid cultures of all three plates were made on 15/8/13, with 16 colonies being selected from each of the three plates (48 liquid cultures in all). All liquid cultures for plate 3 (no visible cultures) had zero growth (as expected) but the liquid cultures for plates one and two were successful. | We have three plates of our gBlocks coding for the green light module, Two show good growth, the third appears to have no growth whatsoever. Liquid cultures of all three plates were made on 15/8/13, with 16 colonies being selected from each of the three plates (48 liquid cultures in all). All liquid cultures for plate 3 (no visible cultures) had zero growth (as expected) but the liquid cultures for plates one and two were successful. | ||
| - | These were Miniprepped this morning, and then run on gels after | + | These were Miniprepped this morning, and then run on gels after being cut with EcoRI and PstI. The gels are shown below. |
| + | |||
| + | ===Gel for Plate 1, colonies 1 through 10=== | ||
| + | |||
| + | Run against 1kb GeneRuler. | ||
| + | |||
| + | [[Image:16.8.13 1.01 to 1.10 green Gblocks.jpg|center||500px|Image: 500 pixels]] | ||
| + | |||
| + | Strangely, the ladder in lane 1 appears to have not worked, but the ladder in lane 12 (far RHS) has. This suggests there may be something wrong with the gel, which is supported by the fact that we can't see <i>any</i> bands for <i>any</i> colonies. Luckily, the results on the other gels are more successful. | ||
| + | |||
| + | ===Gel for Plate 1, colonies 11 through 16=== | ||
| + | |||
| + | Run against 1kb GeneRuler. | ||
| + | |||
| + | [[Image:16.8.13 1.11 to 1.16 green Gblocks.jpg|center||500px|Image: 500 pixels]] | ||
| + | |||
| + | The gel appears to have worked. Each colony shows the same bands | ||
Revision as of 11:24, 29 September 2013
Contents |
Adam + Tom
Miniprep of our complete Cph8 for inertion into CAM plasmid to be sent into the registry.
Miniprep of Complete Cph8 and Phycocyanobilin Operon (part [http://parts.igem.org/Part:BBa_K322122 BBa_K322122])
Realised we had ran out of CAM plasmid, therefore had to transform some more out of the kit plate.
Made transformations of:
TET plasmid
CAM plasmid
KAN plasmid
AMP plasmid
from the kit plates.
Checking gBlocks of Green Light module
We have three plates of our gBlocks coding for the green light module, Two show good growth, the third appears to have no growth whatsoever. Liquid cultures of all three plates were made on 15/8/13, with 16 colonies being selected from each of the three plates (48 liquid cultures in all). All liquid cultures for plate 3 (no visible cultures) had zero growth (as expected) but the liquid cultures for plates one and two were successful.
These were Miniprepped this morning, and then run on gels after being cut with EcoRI and PstI. The gels are shown below.
Gel for Plate 1, colonies 1 through 10
Run against 1kb GeneRuler.
Strangely, the ladder in lane 1 appears to have not worked, but the ladder in lane 12 (far RHS) has. This suggests there may be something wrong with the gel, which is supported by the fact that we can't see any bands for any colonies. Luckily, the results on the other gels are more successful.
Gel for Plate 1, colonies 11 through 16
Run against 1kb GeneRuler.
The gel appears to have worked. Each colony shows the same bands
