Exeter/24 July 2013

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Minipreps using the Qiagen kit

We miniprepped:

  • CcaR
  • Promoter, RBS x 3 replicates
  • CcaS
  • B0034
  • Magenta
  • OmpR promoter
  • OmpR
  • YF1
  • Lambda inverta
  • Fix J
  • Yellow
  • Fix J promoter


Glycerol stocks

We made glycerol stocks of what is above, then stored them at -80oC

For future reference, to get glycerol stocks back as plates, take a loop and streak on gel. Has to be taken from frozen glycerol stocks, which are immediately returned to the freezer.


We then made a gel for digests using ethidium bromide.


NanoDrop from miniprep

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