Exeter/28 August 2013

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(Difference between revisions)
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*RFP (dilution) - 50.4ng/ul
*RFP (dilution) - 50.4ng/ul
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==Digestion==
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{| class="wikitable"
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|-
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! Part !! OmpF (Part A) !! B0034 (Part B) !! AMP Plasmid !! RFP (Control)
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|-
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| DNA || 12.4
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|-
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| Non-nuclease Water ||
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|-
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| NEB Buffer ||
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|-
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| BSA ||
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|-
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| EcoR1 ||
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|-
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| Spe1 ||
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|-
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| Xbal1 ||
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|-
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| Pst1 ||
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|-
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| Dpn1 ||
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|-
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|}

Revision as of 13:46, 28 August 2013

NanoDrop data of the miniprepped, defrosted DNA:

The NanoDrop machine was zero-ed using the elution buffer from the gel extraction kit used.

  • OmpF A - 20.1 ng/ul
  • OmpF B - 9.3ng/ul
  • B0034 - 26.7ng/ul
  • RFP - 198ng/ul

The concentration for OmpF B was too low, so we continued on to the digestion with only OmpF A. The concentration for RFP was too high, so we diluted it.

  • RFP (dilution) - 50.4ng/ul

Digestion

Part OmpF (Part A) B0034 (Part B) AMP Plasmid RFP (Control)
DNA 12.4
Non-nuclease Water
NEB Buffer
BSA
EcoR1
Spe1
Xbal1
Pst1
Dpn1
Retrieved from "http://2013.igem.org/Exeter/28_August_2013"