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Exeter/28 August 2013
From 2013.igem.org
(Difference between revisions)
(→NanoDrop data of the miniprepped, defrosted DNA:) |
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! Part !! OmpF (Part A) !! B0034 (Part B) !! AMP Plasmid !! RFP (Control) | ! Part !! OmpF (Part A) !! B0034 (Part B) !! AMP Plasmid !! RFP (Control) | ||
|- | |- | ||
| - | | DNA || 12.4 | + | | DNA || 12.4 ul || 9.4 ul || 10.0 ul || 5.0 ul |
|- | |- | ||
| - | | Non-nuclease Water || | + | | Non-nuclease Water || 3.6 ul || 6.6 ul || 5.5 ul || 11.0 ul |
|- | |- | ||
| - | | NEB Buffer || | + | | NEB Buffer || 2.5 ul || 2.5 ul || 2.5 ul || 2.5 ul |
|- | |- | ||
| - | | BSA || | + | | BSA || 0.5 ul || 0.5 ul || 0.5 ul || 0.5 ul |
|- | |- | ||
| - | | EcoR1 || | + | | EcoR1 || 0.5 ul || - || 0.5 ul || 0.5 ul |
|- | |- | ||
| - | | Spe1 || | + | | Spe1 || 0.5 || - || - || - |
|- | |- | ||
| - | | Xbal1 || | + | | Xbal1 || - || 0.5 ul || - || - |
|- | |- | ||
| - | | Pst1 || | + | | Pst1 || - || 0.5 ul || 0.5 ul || 0.5 ul |
|- | |- | ||
| - | | Dpn1 || | + | | Dpn1 || - || - || 0.5 ul || - |
|- | |- | ||
|} | |} | ||
Revision as of 13:50, 28 August 2013
NanoDrop data of the miniprepped, defrosted DNA:
The NanoDrop machine was zero-ed using the elution buffer from the gel extraction kit used.
- OmpF A - 20.1 ng/ul
- OmpF B - 9.3ng/ul
- B0034 - 26.7ng/ul
- RFP - 198ng/ul
The concentration for OmpF B was too low, so we continued on to the digestion with only OmpF A. The concentration for RFP was too high, so we diluted it.
- RFP (dilution) - 50.4ng/ul
Digestion
| Part | OmpF (Part A) | B0034 (Part B) | AMP Plasmid | RFP (Control) |
|---|---|---|---|---|
| DNA | 12.4 ul | 9.4 ul | 10.0 ul | 5.0 ul |
| Non-nuclease Water | 3.6 ul | 6.6 ul | 5.5 ul | 11.0 ul |
| NEB Buffer | 2.5 ul | 2.5 ul | 2.5 ul | 2.5 ul |
| BSA | 0.5 ul | 0.5 ul | 0.5 ul | 0.5 ul |
| EcoR1 | 0.5 ul | - | 0.5 ul | 0.5 ul |
| Spe1 | 0.5 | - | - | - |
| Xbal1 | - | 0.5 ul | - | - |
| Pst1 | - | 0.5 ul | 0.5 ul | 0.5 ul |
| Dpn1 | - | - | 0.5 ul | - |
