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Exeter/28 August 2013
From 2013.igem.org
NanoDrop data of the miniprepped, defrosted DNA:
The NanoDrop machine was zero-ed using the elution buffer from the gel extraction kit used.
- OmpF A - 20.1 ng/ul
- OmpF B - 9.3ng/ul
- B0034 - 26.7ng/ul
- RFP - 198ng/ul
The concentration for OmpF B was too low, so we continued on to the digestion with only OmpF A. The concentration for RFP was too high, so we diluted it.
- RFP (dilution) - 50.4ng/ul
Digestion
| Part | OmpF (Part A) | B0034 (Part B) | AMP Plasmid | RFP (Control) |
|---|---|---|---|---|
| DNA | 12.4 | |||
| Non-nuclease Water | ||||
| NEB Buffer | ||||
| BSA | ||||
| EcoR1 | ||||
| Spe1 | ||||
| Xbal1 | ||||
| Pst1 | ||||
| Dpn1 |
