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From 2013.igem.org

Revision as of 10:51, 2 September 2013 (view source) JoshMiddleton (Talk | contribs) (Undo revision 98598 by JoshMiddleton (talk)) ← Older edit		Revision as of 10:51, 2 September 2013 (view source) JoshMiddleton (Talk | contribs) Newer edit →
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	| RBS || 49.1		| RBS || 49.1
	|-		|-
		+
		+
		+
		+	==Digestion==
		+
		+
		+	{| class="wikitable"
		+	|-
		+	! Part !! OmpF (Part A) !! B0034 (Part B) !! AMP Plasmid !! RFP (Control)
		+	|-
		+	| DNA || 12.4 ul || 9.4 ul || 10.0 ul || 5.0 ul
		+	|-
		+	| Non-nuclease Water || 3.6 ul || 6.6 ul || 5.5 ul || 11.0 ul
		+	|-
		+	| NEB Buffer || 2.5 ul || 2.5 ul || 2.5 ul || 2.5 ul
		+	|-
		+	| BSA || 0.5 ul || 0.5 ul || 0.5 ul || 0.5 ul
		+	|-
		+	| EcoR1 || 0.5 ul || - || 0.5 ul || 0.5 ul
		+	|-
		+	| Spe1 || 0.5 || - || - || -
		+	|-
		+	| Xbal1 || - || 0.5 ul || - || -
		+	|-
		+	| Pst1 || - || 0.5 ul || 0.5 ul || 0.5 ul
		+	|-
		+	| Dpn1 || - || - || 0.5 ul || -
		+	|}

---

Revision as of 10:51, 2 September 2013

Minipreps

We miniprepped:

• 1 + 4

• K592011 B

• K864404

• Cph8 #3

• 2 + 4

• K592022 B

• ENvZ no antibiotic

• ENvZ

• Cph8 #5

The liquid cultures of K592011 A and K592022 A did not grow.

NanoDrop from miniprep

Digestion

Part	Concentration (ng/ul)
K592011 B	164.2
B0015	37.7
OmpR promoter	50.7
RBS	49.1

Part	OmpF (Part A)	B0034 (Part B)	AMP Plasmid	RFP (Control)
DNA	12.4 ul	9.4 ul	10.0 ul	5.0 ul
Non-nuclease Water	3.6 ul	6.6 ul	5.5 ul	11.0 ul
NEB Buffer	2.5 ul	2.5 ul	2.5 ul	2.5 ul
BSA	0.5 ul	0.5 ul	0.5 ul	0.5 ul
EcoR1	0.5 ul	-	0.5 ul	0.5 ul
Spe1	0.5	-	-	-
Xbal1	-	0.5 ul	-	-
Pst1	-	0.5 ul	0.5 ul	0.5 ul
Dpn1	-	-	0.5 ul	-