Exeter/29 July 2013

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-== Digest ==
-We followed advice from a PhD student and did:
-* Positive controls of:
-- RFP cut with E and S
-- RFP cut with X and P
-* Negative control of:
-- Just water
-The buffer and BSA were vortexed before use to make sure they were completely defrosted.
-We got fresh purite water each time we needed it.
-We incubated at 37°C for 30 minutes and at 80°C for 20 minutes.

Revision as of 11:43, 6 August 2013