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Exeter/4 July 2013
From 2013.igem.org
(Difference between revisions)
(Created page with " == Morning == * Tried to make clear agar using : 5g NaCl } 15g Tryptone } Makes 1 litre 15g Agar } 5g Soytone } Tryptone, NaCl and so...") |
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== Morning == | == Morning == | ||
| - | * Tried to make clear agar using : | + | * Tried to make 1 litre of clear agar using : |
| - | 5g NaCl | + | 5g NaCl |
| - | 15g Tryptone | + | |
| - | 15g Agar | + | 15g Tryptone |
| - | 5g Soytone | + | |
| + | 15g Agar | ||
| + | |||
| + | 5g Soytone | ||
Tryptone, NaCl and soytone were added into a mixer with a magnetic stirrer. (was a yellow colour). | Tryptone, NaCl and soytone were added into a mixer with a magnetic stirrer. (was a yellow colour). | ||
| Line 15: | Line 18: | ||
Mixture was yellow, not colourless - the experiment was not successful. | Mixture was yellow, not colourless - the experiment was not successful. | ||
Durans put in the autoclave in the evening to let the agar set overnight. | Durans put in the autoclave in the evening to let the agar set overnight. | ||
| - | |||
| - | |||
== Afternoon == | == Afternoon == | ||
*Transformation of competent cells | *Transformation of competent cells | ||
Revision as of 10:38, 15 July 2013
Morning
- Tried to make 1 litre of clear agar using :
5g NaCl
15g Tryptone
15g Agar
5g Soytone
Tryptone, NaCl and soytone were added into a mixer with a magnetic stirrer. (was a yellow colour).
3g of agar were added to two 200ml durans. The Tryptone mix was then added to the durans, filling it up to 200ml.
Mixture was yellow, not colourless - the experiment was not successful. Durans put in the autoclave in the evening to let the agar set overnight.
Afternoon
- Transformation of competent cells
