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Exeter/7 July 2013
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| - | + | ==Making standard liquid cultures of our transformed cells== | |
In each 10 ml Falcon, we need... | In each 10 ml Falcon, we need... | ||
Revision as of 10:36, 22 July 2013
Making standard liquid cultures of our transformed cells
In each 10 ml Falcon, we need...
- 5 ml LB broth
- 5 ul antibiotic (in this case, all of our transformed cells are on the pSB1C3 backbone)
We used a 50 ml conical flask of LB broth which had been autoclaved and added 50 ul of chloramphenicol to make a "stock" to be pipetted into the Falcons.
Stab the chosen colony with a pipette tip and eject into the Falcon tube.
The tubes are incubated overnight in a spinning 37oC incubator.
