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Exeter/7 July 2013
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(Created page with "'''Making standard liquid cultures of our transformed cells''' In each 10ml Falcon, we need... - 5ml LB broth - 5ul antibiotic (in this case, all of our transformed cells are ...")
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(Created page with "'''Making standard liquid cultures of our transformed cells''' In each 10ml Falcon, we need... - 5ml LB broth - 5ul antibiotic (in this case, all of our transformed cells are ...")
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Revision as of 14:09, 15 July 2013
Making standard liquid cultures of our transformed cells
In each 10ml Falcon, we need...
- 5ml LB broth
- 5ul antibiotic (in this case, all of our transformed cells are on the pSB1C3 backbone)
We used a 50ml conical flask of LB broth which had been autoclaved and added 50ul of chloramphenicol to make a "stock" to be pipetted into the Falcons.
Stab the chosen colony with a pipette tip and eject into the Falcon tube.
The tubes are incubated overnight in a spinning 37oC incubator.
