Exeter/7 July 2013

From 2013.igem.org

Making standard liquid cultures of our transformed cells

In each 10 ml Falcon, we need...

- 5 ml LB broth

- 5 ul antibiotic (in this case, all of our transformed cells are on the pSB1C3 backbone)

We used a 50 ml conical flask of LB broth which had been autoclaved and added 50 ul of chloramphenicol to make a "stock" to be pipetted into the Falcons.

Stab the chosen colony with a pipette tip and eject into the Falcon tube.

The tubes are incubated overnight in a spinning 37oC incubator.