Team:Alberta/FAQ

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      <h3><font color="#A80000"><font size="6">The Littlest Mapmaker</font></font></h3>
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      <h4><i><font size="2">"Exploration into the world of <font color="#A80000">DNA Computing</font>"<br/>
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        Team Alberta: University of Alberta</font></i></h4>
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             <li><a href="/Team:Alberta/Team">Roster</a></li>
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             <li><a href="https://igem.org/Team.cgi?year=2013&team_name=Alberta">Official Profile</a></li>
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          <span class="saying">Welcome to the Team Alberta Wiki!
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          <h5>Other Sections</h5>
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            <a href="/Team:Alberta/SafetyForm"><p>Safety Form</p></a>
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             <a href="/Team:Alberta/FAQ" class="active"><p>FAQ</p></a>
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              For visitors: this site is currently under construction. Please contact our Student Liason, Dawson at                  daocun@ualberta.ca, for more information on our current project and how to support us!
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             <a href="/Team:Alberta/Resources"><p>Resources</p></a>
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         <p class="content-title">FAQ</p>
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         <p class="content-title"><img src="/wiki/images/thumb/b/b0/Public_members.png/463px-Public_members.png " height="120"></img>  FAQ</p>
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Latest revision as of 03:53, 29 October 2013


FAQ

Doesn’t E. coli cause food poisoning? If E. coli is so dangerous, why are they being used in labs?

  • Normal E. coli colonize the lower intestine of humans, while producing Vitamin K and protecting infection from other harmful bacteria.
  • Only some strains of E. coli cause food poisoning, but our group is using a harmless, nonpathogenic strain. This train is engineered to contain mutations that would not allow the E. coli to survive outside the lab without particular nutrients.
  • E. coli provides a well-studied and well-known model system to test and troubleshoot new trials on.

Reference: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1728375/

What if plasmids containing antibiotic resistance genes were released into the environment despite precautions?

  • These genes are used in synthetic biology as selection tools.
  • There are lower chances of gene uptake by wild bacteria compared to lab-strain bacteria which have perforated membranes.
  • In the case that wild bacteria did uptake the antibiotic resistance gene, that gene would eventually be lost in the population without exposure to the antibiotic, because it is then a disadvantage for the bacteria to carry the unused plasmids. (Evidence observed in past with the decline of specific antibiotic resistance after the human population limited that antibiotic).