http://2013.igem.org/wiki/index.php?title=Team:BIOSINT_Mexico/Modeling&feed=atom&action=historyTeam:BIOSINT Mexico/Modeling - Revision history2024-03-29T10:08:24ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:BIOSINT_Mexico/Modeling&diff=241566&oldid=prevGabyqz at 03:45, 28 September 20132013-09-28T03:45:19Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The graph represent an exponential growth of luminescent that at the same time is restricted the own GFP´s spectrum which only gives green color from 395nm to 475nm, also external factors like the medium in which bacteria growth because is limited.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The graph represent an exponential growth of luminescent that at the same time is restricted the own GFP´s spectrum which only gives green color from 395nm to 475nm, also external factors like the medium in which bacteria growth because is limited.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Recovered on september 5 from 2013 with an url of http://dwb.unl.edu/Teacher/NSF/C08/C08Links/pps99.cryst.bbk.ac.uk/projects/gmocz/gfp.htm</ins></div></td></tr>
</table>Gabyqzhttp://2013.igem.org/wiki/index.php?title=Team:BIOSINT_Mexico/Modeling&diff=241400&oldid=prevGabyqz at 03:42, 28 September 20132013-09-28T03:42:57Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>What we pretend with this mathematic model is to represent in a numerical way, the expression of the protein in the cell according with the emission that each concentration of GFP provides. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>What we pretend with this mathematic model is to represent in a numerical way, the expression of the protein in the cell according with the emission that each concentration of GFP provides. </div></td></tr>
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</table>Gabyqzhttp://2013.igem.org/wiki/index.php?title=Team:BIOSINT_Mexico/Modeling&diff=241364&oldid=prevGabyqz at 03:42, 28 September 20132013-09-28T03:42:23Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <del class="diffchange diffchange-inline">(</del>y=a/(1+be<del class="diffchange diffchange-inline">)</del>^(-ct))</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> y=a/(1+be^(-ct))</div></td></tr>
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</table>Gabyqzhttp://2013.igem.org/wiki/index.php?title=Team:BIOSINT_Mexico/Modeling&diff=241322&oldid=prevGabyqz at 03:41, 28 September 20132013-09-28T03:41:39Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>What we pretend with this mathematic model is to represent in a numerical way, the expression of the protein in the cell according with the emission that each concentration of GFP provides. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>What we pretend with this mathematic model is to represent in a numerical way, the expression of the protein in the cell according with the emission that each concentration of GFP provides. </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>(y=a/(1+be)^(-ct))</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins></div></td></tr>
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</table>Gabyqzhttp://2013.igem.org/wiki/index.php?title=Team:BIOSINT_Mexico/Modeling&diff=241264&oldid=prevGabyqz at 03:40, 28 September 20132013-09-28T03:40:45Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>What we pretend with this mathematic model is to represent in a numerical way, the expression of the protein in the cell according with the emission that each concentration of GFP provides. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>What we pretend with this mathematic model is to represent in a numerical way, the expression of the protein in the cell according with the emission that each concentration of GFP provides. </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We used linear regression in order to get the values of the constants, using statistical methods that also are applied in the equation. The slope is the value given to b and the intersection is the value for a.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We used linear regression in order to get the values of the constants, using statistical methods that also are applied in the equation. The slope is the value given to b and the intersection is the value for a.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The graph represent an exponential growth of luminescent that at the same time is restricted the own GFP´s spectrum which only gives green color from 395nm to 475nm, also external factors like the medium in which bacteria growth because is limited.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The graph represent an exponential growth of luminescent that at the same time is restricted the own GFP´s spectrum which only gives green color from 395nm to 475nm, also external factors like the medium in which bacteria growth because is limited.</div></td></tr>
</table>Gabyqzhttp://2013.igem.org/wiki/index.php?title=Team:BIOSINT_Mexico/Modeling&diff=241225&oldid=prevGabyqz at 03:40, 28 September 20132013-09-28T03:40:19Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>GFP tends to get fluorescents at the 395 nm because of the excitation of the protein this extend to the 475 nm where the green light is still visible, so we used this information in order to determine an equation that represents the exponential growth of the luminescent.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>GFP tends to get fluorescents at the 395 nm because of the excitation of the protein this extend to the 475 nm where the green light is still visible, so we used this information in order to determine an equation that represents the exponential growth of the luminescent.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>What we pretend with this mathematic model is to represent in a numerical way, the expression of the protein in the cell according with the emission that each concentration of GFP provides. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>What we pretend with this mathematic model is to represent in a numerical way, the expression of the protein in the cell according with the emission that each concentration of GFP provides. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> (y=a/(1+be)^(-ct))</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">\\</ins>(y=a/(1+be)^(-ct))</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">X Y a= 385</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">0 395 b= 10</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">1 405 </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">2 415 </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">3 425 </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">4 435 </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">5 445 </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">6 455 </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">7 465 </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">8 475 </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We used linear regression in order to get the values of the constants, using statistical methods that also are applied in the equation. The slope is the value given to b and the intersection is the value for a.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We used linear regression in order to get the values of the constants, using statistical methods that also are applied in the equation. The slope is the value given to b and the intersection is the value for a.</div></td></tr>
</table>Gabyqzhttp://2013.igem.org/wiki/index.php?title=Team:BIOSINT_Mexico/Modeling&diff=241016&oldid=prevGabyqz at 03:37, 28 September 20132013-09-28T03:37:30Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> (y=a/(1+be)^(-ct))</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> (y=a/(1+be)^(-ct))</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>X Y a= 385</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>X <ins class="diffchange diffchange-inline"> </ins>Y <ins class="diffchange diffchange-inline"> </ins>a= <ins class="diffchange diffchange-inline"> </ins>385</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>0 395 b= 10</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>0 <ins class="diffchange diffchange-inline"> </ins>395 b= <ins class="diffchange diffchange-inline"> </ins>10</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>1 405 </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>1 <ins class="diffchange diffchange-inline"> </ins>405 </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>2 415 </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>2 <ins class="diffchange diffchange-inline"> </ins>415 </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>3 425 </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>3 <ins class="diffchange diffchange-inline"> </ins>425 </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>4 435 </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>4 <ins class="diffchange diffchange-inline"> </ins>435 </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>5 445 </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>5 <ins class="diffchange diffchange-inline"> </ins>445 </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>6 455 </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>6 <ins class="diffchange diffchange-inline"> </ins>455 </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>7 465 </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>7 <ins class="diffchange diffchange-inline"> </ins>465 </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>8 475 </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>8 <ins class="diffchange diffchange-inline"> </ins>475 </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Gabyqzhttp://2013.igem.org/wiki/index.php?title=Team:BIOSINT_Mexico/Modeling&diff=240928&oldid=prevGabyqz at 03:36, 28 September 20132013-09-28T03:36:25Z<p></p>
<table style="background-color: white; color:black;">
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<col class='diff-content' />
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:36, 28 September 2013</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 8:</td>
<td colspan="2" class="diff-lineno">Line 8:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>GFP tends to get fluorescents at the 395 nm because of the excitation of the protein this extend to the 475 nm where the green light is still visible, so we used this information in order to determine an equation that represents the exponential growth of the luminescent.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>GFP tends to get fluorescents at the 395 nm because of the excitation of the protein this extend to the 475 nm where the green light is still visible, so we used this information in order to determine an equation that represents the exponential growth of the luminescent.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>What we pretend with this mathematic model is to represent in a numerical way, the expression of the protein in the cell according with the emission that each concentration of GFP provides.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>What we pretend with this mathematic model is to represent in a numerical way, the expression of the protein in the cell according with the emission that each concentration of GFP provides. <ins class="diffchange diffchange-inline"> </ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>(y=a/(1+be)^(-ct))</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins>(y=a/(1+be)^(-ct))</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>X Y a= 385</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>X Y a= 385</div></td></tr>
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<td colspan="2" class="diff-lineno">Line 21:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>7 465 </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>7 465 </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>8 475 </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>8 475 </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We used linear regression in order to get the values of the constants, using statistical methods that also are applied in the equation. The slope is the value given to b and the intersection is the value for a.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We used linear regression in order to get the values of the constants, using statistical methods that also are applied in the equation. The slope is the value given to b and the intersection is the value for a.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>y=a/(1+be^(-ct))</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>y=a/(1+be^(-ct))</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>be^(-ct)=a/y-1</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>be^(-ct)=a/y-1</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>ln(b)-ct=ln(a/y)-ln(1)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>ln(b)-ct=ln(a/y)-ln(1)</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>c=(ln)(b)-ln(a)-ln(y))/t</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>c=(ln)(b)-ln(a)-ln(y))/t</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[File:<del class="diffchange diffchange-inline">MSP97441g4b054f29a477d400005ab59eff9036he24</del>.<del class="diffchange diffchange-inline">gif</del>\\]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[File:<ins class="diffchange diffchange-inline">Plot</ins>.<ins class="diffchange diffchange-inline">jpg</ins>\\]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The graph represent an exponential growth of luminescent that at the same time is restricted the own GFP´s spectrum which only gives green color from 395nm to 475nm, also external factors like the medium in which bacteria growth because is limited.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The graph represent an exponential growth of luminescent that at the same time is restricted the own GFP´s spectrum which only gives green color from 395nm to 475nm, also external factors like the medium in which bacteria growth because is limited.</div></td></tr>
</table>Gabyqzhttp://2013.igem.org/wiki/index.php?title=Team:BIOSINT_Mexico/Modeling&diff=239629&oldid=prevGabyqz at 03:20, 28 September 20132013-09-28T03:20:11Z<p></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:20, 28 September 2013</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 9:</td>
<td colspan="2" class="diff-lineno">Line 9:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>GFP tends to get fluorescents at the 395 nm because of the excitation of the protein this extend to the 475 nm where the green light is still visible, so we used this information in order to determine an equation that represents the exponential growth of the luminescent.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>GFP tends to get fluorescents at the 395 nm because of the excitation of the protein this extend to the 475 nm where the green light is still visible, so we used this information in order to determine an equation that represents the exponential growth of the luminescent.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>What we pretend with this mathematic model is to represent in a numerical way, the expression of the protein in the cell according with the emission that each concentration of GFP provides.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>What we pretend with this mathematic model is to represent in a numerical way, the expression of the protein in the cell according with the emission that each concentration of GFP provides.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">〖y</del>=a/(1+<del class="diffchange diffchange-inline">be〗</del>^(-ct))</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">(y</ins>=a/(1+<ins class="diffchange diffchange-inline">be)</ins>^(-ct))</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>X Y a= 385</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>X Y a= 385</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>y=a/(1+be^(-ct) )</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>y=a/(1+be^(-ct))</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>be^(-ct)=a/y-1</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>be^(-ct)=a/y-1</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">ln</del>(b)-ct=<del class="diffchange diffchange-inline">ln</del>(a/y)-<del class="diffchange diffchange-inline">ln</del>(1)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">ln</ins>(b)-ct=<ins class="diffchange diffchange-inline">ln</ins>(a/y)-<ins class="diffchange diffchange-inline">ln</ins>(1)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>c=<del class="diffchange diffchange-inline">〖</del>(<del class="diffchange diffchange-inline">ln〗</del>(b)-<del class="diffchange diffchange-inline">ln</del>(a)-<del class="diffchange diffchange-inline">ln</del>(y))/t</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>c=(<ins class="diffchange diffchange-inline">ln)</ins>(b)-<ins class="diffchange diffchange-inline">ln</ins>(a)-<ins class="diffchange diffchange-inline">ln</ins>(y))/t</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[File:MSP97441g4b054f29a477d400005ab59eff9036he24.gif\\]]</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The graph represent an exponential growth of luminescent that at the same time is restricted the own GFP´s spectrum which only gives green color from 395nm to 475nm, also external factors like the medium in which bacteria growth because is limited.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The graph represent an exponential growth of luminescent that at the same time is restricted the own GFP´s spectrum which only gives green color from 395nm to 475nm, also external factors like the medium in which bacteria growth because is limited.</div></td></tr>
</table>Gabyqzhttp://2013.igem.org/wiki/index.php?title=Team:BIOSINT_Mexico/Modeling&diff=239430&oldid=prevGabyqz at 03:17, 28 September 20132013-09-28T03:17:14Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:17, 28 September 2013</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 7:</td>
<td colspan="2" class="diff-lineno">Line 7:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">If you choose </del>to <del class="diffchange diffchange-inline">include </del>a <del class="diffchange diffchange-inline">'''Modeling''' page</del>, <del class="diffchange diffchange-inline">please write about your modeling adventures here</del>. <del class="diffchange diffchange-inline"> This </del>is <del class="diffchange diffchange-inline">not necessary but it may </del>be a <del class="diffchange diffchange-inline">nice list </del>to <del class="diffchange diffchange-inline">include</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">GFP tends </ins>to <ins class="diffchange diffchange-inline">get fluorescents at the 395 nm because of the excitation of the protein this extend to the 475 nm where the green light is still visible, so we used this information in order to determine an equation that represents the exponential growth of the luminescent.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">What we pretend with this mathematic model is to represent in </ins>a <ins class="diffchange diffchange-inline">numerical way</ins>, <ins class="diffchange diffchange-inline">the expression of the protein in the cell according with the emission that each concentration of GFP provides</ins>.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">〖y=a/(1+be〗^(-ct))</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">X Y a= 385</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">0 395 b= 10</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">1 405 </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">2 415 </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">3 425 </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">4 435 </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">5 445 </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">6 455 </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">7 465 </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">8 475 </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">We used linear regression in order to get the values of the constants, using statistical methods that also are applied in the equation. The slope </ins>is <ins class="diffchange diffchange-inline">the value given to b and the intersection is the value for a.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">y=a/(1+</ins>be<ins class="diffchange diffchange-inline">^(-ct) )</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">be^(-ct)=</ins>a<ins class="diffchange diffchange-inline">/y-1</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">ln(b)-ct=ln(a/y)-ln(1)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">c=〖(ln〗(b)-ln(a)-ln(y))/t</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">The graph represent an exponential growth of luminescent that at the same time is restricted the own GFP´s spectrum which only gives green color from 395nm </ins>to <ins class="diffchange diffchange-inline">475nm, also external factors like the medium in which bacteria growth because is limited</ins>.</div></td></tr>
</table>Gabyqz